Methods Article

Biochimica e High Throughput valutazione microscopica di massa grassa in

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20.5K Views

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16:07 min

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March 30th, 2013

DOI :

10.3791/50180-v

March 30th, 2013

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Elizabeth C. Pino¹, Christopher M. Webster¹, Christopher E. Carr², Alexander A. Soukas¹

¹Center for Human Genetic Research and Department of Medicine, Massachusetts General Hospital and Harvard Medical School, ²Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology

Riepilogo

Capitoli in questo video

0:05

Title

2:06

Stamp Frozen Library Plates onto Rectangular Amp/Tet LB Agar Plates

3:16

Grow and Seed RNAi Bacterial Clones and Add Worms to RNAi Plates

5:49

Fix and Stain Worms in Nile Red and Image on a High-throughput Scope

7:22

Wash Worms and Image on a High-throughput Microscope

8:31

Biochemical Lipid Determination Using Solid Phase Extraction and Gas Chromatography-mass Spectrometry

12:02

Representative Fat Mass Content Results

15:39

Conclusion

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