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Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

DOI :

10.3791/57042-v

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12:04 min

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May 9th, 2018

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12,982 Views

Vanessa Neuhaus^1*, Olga Danov^1*, Sebastian Konzok¹, Helena Obernolte¹, Susann Dehmel¹, Peter Braubach², Danny Jonigk², Hans-Gerd Fieguth³, Patrick Zardo⁴, Gregor Warnecke⁴, Christian Martin⁵, Armin Braun^1,6, Katherina Sewald¹

¹Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, ²Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ³Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), ⁴Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), ⁵Institute of Pharmacology and Toxicology, RWTH Aachen University, ⁶Institute for Immunology, Hannover Medical School

Chapters

In view of the 3Rs principle, respiratory models as alternatives to animal studies are evolving. Especially for risk assessment of respiratory substances, there is a lack of appropriate assays. Here, we describe the use of human precision-cut lung slices for the assessment of airborne substances.

Tags

Human Precision cut Lung Slices

Cytotoxicity

Immunomodulatory Effects

Physiological Response

Respiratory Diseases

Fresh Human Tissue

Human Cell cell Interactions

Pathomechanism

Respiratory Disease

Species specific Applications

Reliable Infrastructure

Cannulation Of Trachea

Silicone Tube

Clamp Fixation

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