JoVE Journal

Neuroscience

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A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

2.3K Views

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06:34 min

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June 2nd, 2023

DOI :

10.3791/65228-v

June 2nd, 2023

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Robert J. Pendry¹^,², Lilyana D. Quigley¹^,², Lenora J. Volk¹^,³^,⁴, Brad E. Pfeiffer¹^,³

¹Department of Neuroscience, UT Southwestern Medical Center, ²Neuroscience Graduate Program, UT Southwestern Medical Center, ³O’Donnell Brain Institute, UT Southwestern Medical Center, ⁴Department of Psychiatry, UT Southwestern Medical Center

Summary

Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.

Explore More Videos

Lightweight Drive Implant

Chronic Tetrode Recordings

Network Activity

In Vivo Electrophysiology

Silicon Probe Technology

Theta Oscillation

Neurodevelopmental Disorders

Autism Spectrum Disorders

Spatial Distribution

Recording Challenges

Functional Communication

Circuit Dynamics

Chapters in this video

0:00

Introduction

1:44

Micro‐Drive Design and Construction

4:57

Surgical Implantation of the Micro‐Drive on the Mouse Skull

5:43

Recovery after Micro‐Drive Implantation

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