Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

974 Views

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07:55 min

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June 30th, 2023

DOI :

10.3791/65650-v

June 30th, 2023

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Gabriel R. Martins*¹^,², Felipe L. Brum*¹^,²^,³, Davi Marconi Miranda Carvalho da Silva*¹^,², Livia C. Barbosa³, Ronaldo Mohana-Borges³, Ayla Sant'Ana da Silva¹^,²

¹Laboratório de Biocatálise (LABIC), Instituto Nacional de Tecnologia, ²Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, ³Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro

Summary

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Mass Spectrometry

Sample Preparation

Biological Tissue

Molecular Mapping

Hard Palm Seeds

Imaging Analysis

Oligosaccharides

Seed Development

Chemical Compounds

Botanical Materials

Physiological Information

Chapters in this video

0:00

Introduction

2:18

Seed Sectioning Protocol and Matrix Deposition Technique for MALDI‐IMS Analysis of Hard Palm Seeds

4:39

Image Acquisition and MALDI‐IMS Analysis of Hard Palm Seeds

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