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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

DOI :

10.3791/52313-v

8:14 min

December 5th, 2014

December 5th, 2014

17,569 Views

1Department of Chemistry and Biochemistry, University of Notre Dame, 2Harper Cancer Research Institute, University of Notre Dame

Immortalized cancer cell lines can be grown as 3D cell cultures, a valuable model for biological research. This protocol describes mass spectrometry imaging of 3D cell cultures, including improvements in the sample preparation platform. The goal of this protocol is to instruct users to prepare 3D cell cultures for mass spectrometry imaging analysis.

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Keywords 3D Cell Culture

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