Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

DOI :

10.3791/55254-v

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March 23rd, 2017

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10,512 Views

Sarah Caughlin¹, Dae Hee Park¹, Ken K-C Yeung², David F. Cechetto¹, Shawn N. Whitehead¹

¹Department of Anatomy and Cell Biology, The University of Western Ontario, ²Department of Chemistry and Biochemistry, The University of Western Ontario

Chapters

A protocol for the sublimation of DAN matrix onto rat brain tissue for the detection of gangliosides using MALDI Imaging Mass Spectrometry is presented.

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