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Kasetsart University

5 ARTICLES PUBLISHED IN JoVE

Environment

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR

Pamela Nicholson¹, Pattarasuda Rawiwan², Win Surachetpong²

¹Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, ²Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

This protocol diagnoses Tilapia Lake Virus (TiLV) in tilapia tissues using RT-PCR methodologies. The entire method is described from tissue dissection to total RNA extraction, followed by cDNA synthesis and detection of TiLV by either conventional PCR or quantitative PCR using dsDNA binding a fluorescent binding dye.

Immunology and Infection

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus

Theerawut Phusantisampan¹, Pattarasuda Rawiwan^2,3, Sri Rajiv Kumar Roy², Malinee Sriariyanun⁴, Win Surachetpong^2,3

¹Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), ²Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, ³Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, ⁴Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.

Bioengineering

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering

Sougata Ghosh^1,2,3, Tanay Bhagwat³, Rohini Kitture⁴, Sirikanjana Thongmee¹, Thomas J. Webster^3,5,6,7

¹Department of Physics, Faculty of Science, Kasetsart University, ²Department of Microbiology, School of Science, RK. University, ³Department of Chemical Engineering, Northeastern University, ⁴Defence Institute of Advanced Technology Campus, Navyukti Innovations Pvt. Ltd., ⁵Department of Biomedical Engineering, Hebei University of Technology, ⁶Department of Chemistry, Saveetha School of Engineering, Institute of Medical and Technical Science, Saveetha University, ⁷UFPI - Universidade Federal do Piauí

Novel nanocomposites of graphene nanoribbons and hydroxyapatite nanoparticles were prepared using solution-phase synthesis. These hybrids when employed in bioactive scaffolds can exhibit potential applications in tissue engineering and bone regeneration.

Biology

Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs

Mohammad Asmat-Ullah¹, Rusydi Rozaimi¹, Hanafiah Fazhan^1,2,3, Alexander Chong Shu-Chien^2,4, Youji Wang⁵, Khor Waiho^1,2,3

¹Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, ²Centre for Chemical Biology, Universiti Sains Malaysia, ³Department of Aquaculture, Faculty of Fisheries, Kasetsart University, ⁴School of Biological Sciences, Universiti Sains Malaysia, ⁵International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University

Two eyestalk ablation protocols (i.e., cauterization and surgery approaches) were performed on anesthetized female crabs. The eyestalk ablation of mud crabs hastened the ripening of ovaries without decreasing the survival rate.

Biology

Surgical Technique of the 3-Dimensional-printed Personalized Hip Implant for the Treatment of Canine Hip Dysplasia

Irin Kwananocha^1,2, Femke Verseijden¹, Seyed A Kamali¹, Joëll Magré³, Koen Willemsen³, Jacobine CM Schouten⁴, Daniela Salvatori⁴, Marianna A Tryfonidou¹, Björn P Meij¹

¹Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, ²Research and Academic Service, Faculty of Veterinary Medicine, Kasetsart University, ³Department of Orthopedics, University Medical Center Utrecht, ⁴Department of Clinical Sciences, Anatomy and Physiology, Faculty of Veterinary Medicine, Utrecht University

This work describes a novel surgical technique for extracapsular implantation of a personalized, 3-dimensional-printed, joint-preserving implant. This novel treatment aims to restore hip stability in young adult dogs suffering from hip dysplasia with laxity by uniquely reproducing the anatomical shape of the acetabular rim of the hip joint.