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southwest medical university

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

A Pilot Study on the Repetitive Transcranial Magnetic Stimulation of Aβ and Tau Levels in Rhesus Monkey Cerebrospinal Fluid

Ling-Yi Liao^1,2,3, Ying-Qian Zhang⁴, Benson Wui-Man Lau⁵, Qiong Wu⁶, Zi-Yan Fan^1,3, Qiang Gao^1,3, Zhi-Hui Zhong^6,7

¹Department of Rehabilitation Medicine, West China Hospital, Sichuan University, ²Department of Rehabilitation Medicine, Daping Hospital, Army Medical University, ³Key Laboratory of Rehabilitation Medicine in Sichuan Province, West China Hospital, Sichuan University, ⁴Department of physiology, Southwest Medical University, ⁵Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, ⁶Laboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, ⁷Sichuan Kangcheng Biotech Co., Inc.

Here, we describe the procedure for a pilot study to explore the effect of repetitive transcranial magnetic stimulation with different frequencies (1 Hz/20 Hz/40 Hz) on Aβ and tau metabolism in rhesus monkey cerebrospinal fluid.

Biology

Dual-Dye Optical Mapping of Hearts from RyR2^R2474S Knock-In Mice of Catecholaminergic Polymorphic Ventricular Tachycardia

Yangpeng Li^*1,2, Jun Yang^*1, Rui Zhang¹, Tangting Chen¹, Shiyu Zhang¹, Yuqing Zheng¹, Qiang Wen³, Tao Li¹, Xiaoqiu Tan^1,2, Ming Lei^1,4, Xianhong Ou¹

¹Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, ²Department of Cardiology, the Affiliated Hospital of Southwest Medical University, ³Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, ⁴Department of Pharmacology, University of Oxford

This protocol introduces dual-dye optical mapping of mouse hearts obtained from wild-type and knock-in animals affected by catecholaminergic polymorphic ventricular tachycardia, including electrophysiological measurements of transmembrane voltage and intracellular Ca²⁺ transients with high temporal and spatial resolution.

Medicine

Immunomagnetic Isolation of the Vascular Wall-Resident CD34⁺ Stem Cells from Mice

Chuting Han¹, Cheng Xie¹, Qingya Dang¹, Xiaolin Zhang¹, Chunshu Li¹, Yan Yang¹, Jun Cheng¹, Pengyun Li¹

¹Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University

This study has established a stable and efficient method for the isolation, culture, and functional determination of vascular wall-resident CD34⁺ stem cells (CD34⁺ VW-SCs). This easy-to-follow and time-effective isolation method can be utilized by other investigators to study the potential mechanisms involved in cardiovascular diseases.