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University of Maryland Baltimore

4 ARTICLES PUBLISHED IN JoVE

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Biochemistry

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans
Jessica A. Espino 1, Lisa M. Jones 1
1Department of Pharmaceutical Sciences, University of Maryland

In vivo fast photochemical oxidation of proteins (IV-FPOP) is a hydroxyl radical protein footprinting technique that allows for mapping of protein structure in their native environment. This protocol describes the assembly and set-up of the IV-FPOP microfluidic flow system.

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Biochemistry

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins
Emily E. Chea 1, Aimee Rinas 2, Jessica A. Espino 1, Lisa M. Jones 1
1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2AIT Bioscience

Here, we characterize protein structure and interaction sites in living cells using a protein footprinting technique termed in-cell fast photochemical oxidation of proteins (IC-FPOP).

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JoVE Journal

Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
Scot R. Weinberger 1, Emily E. Chea 1, Joshua S. Sharp 1,2,3, Sandeep K. Misra 2
1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, 3Department of Chemistry and Biochemistry, University of Mississippi

This protocol presents a method to use inline radical dosimetry and a plasma light source to perform flash oxidation protein footprinting. This method replaces the hazardous UV laser to simplify and improve the reproducibility of fast photochemical oxidation of protein studies.

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Biochemistry

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
Danté Johnson 1, Benjamin Punshon-Smith 2, Jessica A. Espino 1, Anne Gershenson 3, Lisa M. Jones 1
1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2Technology Research Center, University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology, University of Massachusetts

A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP).

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