Accedi

University of Maryland Baltimore

4 ARTICLES PUBLISHED IN JoVE

Biochemistry

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

¹Department of Pharmaceutical Sciences, University of Maryland

In vivo fast photochemical oxidation of proteins (IV-FPOP) is a hydroxyl radical protein footprinting technique that allows for mapping of protein structure in their native environment. This protocol describes the assembly and set-up of the IV-FPOP microfluidic flow system.

Biochemistry

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Emily E. Chea¹, Aimee Rinas², Jessica A. Espino¹, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences, University of Maryland Baltimore, ²AIT Bioscience

Here, we characterize protein structure and interaction sites in living cells using a protein footprinting technique termed in-cell fast photochemical oxidation of proteins (IC-FPOP).

JoVE Journal

Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins

Scot R. Weinberger¹, Emily E. Chea¹, Joshua S. Sharp^1,2,3, Sandeep K. Misra²

¹GenNext Technologies Inc., ²Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, ³Department of Chemistry and Biochemistry, University of Mississippi

This protocol presents a method to use inline radical dosimetry and a plasma light source to perform flash oxidation protein footprinting. This method replaces the hazardous UV laser to simplify and improve the reproducibility of fast photochemical oxidation of protein studies.

Biochemistry

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

Danté Johnson¹, Benjamin Punshon-Smith², Jessica A. Espino¹, Anne Gershenson³, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences, University of Maryland Baltimore, ²Technology Research Center, University of Maryland Baltimore County, ³Department of Biochemistry and Molecular Biology, University of Massachusetts

A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP).