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University of Duisburg/Essen

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy

Mike Hasenberg¹, Anja Köhler¹, Susanne Bonifatius¹, Andreas Jeron², Matthias Gunzer¹

¹Institute for Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, ²Department of Immunoregulation, Helmholtz Center for Infection Research

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

Medicine

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Linda Männ¹, Anika Klingberg², Matthias Gunzer², Mike Hasenberg³

¹Department of Translational Skin Cancer Research, University of Duisburg/Essen, ²Institute for Experimental Immunology and Imaging, University of Duisburg/Essen, ³Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen

Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45⁺ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.