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University of Duisburg/Essen

2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
Mike Hasenberg 1, Anja Köhler 1, Susanne Bonifatius 1, Andreas Jeron 2, Matthias Gunzer 1
1Institute for Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation, Helmholtz Center for Infection Research

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

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Medicine

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy
Linda Männ 1, Anika Klingberg 2, Matthias Gunzer 2, Mike Hasenberg 3
1Department of Translational Skin Cancer Research, University of Duisburg/Essen, 2Institute for Experimental Immunology and Imaging, University of Duisburg/Essen, 3Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen

Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.

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