To begin preparing the paraffin sections of the kidney and Peyer's patch isolated from properly-anesthetized IgA neuropathy model mouse, first fix one Peyer's patch or three-millimeter-thick kidney tissues with 4%paraformaldehyde for 24 hours. Then dehydrate them using a gradient of ethanol and xylene before washing them with water. Submerge the tissues in wax for two hours before sealing and freezing them.
Next, cut four-micron-thick Peyer's patch sections or two-micron-thick kidney sections and place them in warm water to unfold. Using a clean glass slide, gently pick up the unfolded slices and incubate them in a constant temperature oven at 40 degrees Celsius for one hour before staining.
