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Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

11.3K Views

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09:38 min

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May 31st, 2011

DOI :

10.3791/2645-v

May 31st, 2011

•
1Department of Cell and Molecular Biology, Northwestern University

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Small GTPases
Microinjection
Plasmids
Polarized Epithelial Cells
Apical Domain
Basolateral Domain
Tight Junctions
MDCK Cells
Cell Polarity
Ras Superfamily
RalA
Cdc42
Rab8
Rab10
Rab13
GDP bound State
GTP bound State
Nucleotide Binding Regions
Dominant Negative
Dominant Active Alleles
Overexpression
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Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

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