ウェルカムトラストからの資金は、親切（助成番号085245/Z/08/Z）認識されています。ノッティンガムナノテクノロジーとナノサイエンスセンターは、親切に、この装置を資金調達のためのラマンシステムへのアクセスを与えるために、イーストミッドランド開発庁のために承認されます。

<ol>
	<li>Hook, A. L., Anderson, D. G., Langer, R., Williams, P., Davies, M. C., Alexander, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+throughput+methods+applied+in+biomaterial+development+and+discovery.">High throughput methods applied in biomaterial development and discovery.</a> Biomaterials. 31, 187-198 (2010).</li><li>Anderson, D. G., Levenberg, S., Langer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanoliter-scale+synthesis+of+arrayed+biomaterials+and+application+to+human+embryonic+stem+cells.">Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells.</a> Nature Biotechnology. 22, 863-866 (2004).</li><li>Mei, Y., Saha, K., Bogatyrev, S. R., Yang, J., Hook, A. L., Kalcioglu, Z. I., Cho, S. W., Mitalipova, M., Pyzocha, N., Rojas, F., Van Vliet, K. J., Davies, M. C., Alexander, M. R., Langer, R., Jaenisch, R., Anderson, D. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Combinatorial+development+of+biomaterials+for+clonal+growth+of+human+pluripotent+stem+cells.">Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells.</a> Nature Materials. 9, 768-778 (2010).</li><li>Pernagallo, S., Wu, M., Gallagher, M. P., Bradley, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colonising+new+frontiers-microarrays+reveal+biofilm+modulating+polymers.">Colonising new frontiers-microarrays reveal biofilm modulating polymers.</a> Journal of Materials Chemistry. 21, 96-101 (2011).</li><li>Yang, J., Mei, Y., Hook, A. L., Taylor, M., Urquhart, A. J., Bogatyrev, S. R., Langer, R., Anderson, D. G., Davies, M. C., Alexander, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polymer+surface+functionalities+that+control+human+embryoid+body+cell+adhesion+revealed+by+high+throughput+surface+characterization+of+combinatorial+material+microarrays.">Polymer surface functionalities that control human embryoid body cell adhesion revealed by high throughput surface characterization of combinatorial material microarrays.</a> Biomaterials. 31, 8827-8838 (2010).</li><li>Urquhart, A. J., Anderson, D. G., Taylor, M., Alexander, M. R., Langer, R., Davies, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+throughput+surface+characterisation+of+a+combinatorial+material+library.">High throughput surface characterisation of a combinatorial material library.</a> Advanced Materials. 19, 2486-2491 (2007).</li><li>Taylor, M., Urquhart, A. J., Zelzer, M., Davies, M. C., Alexander, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Picoliter+water+contact+angle+measurement+on+polymers.">Picoliter water contact angle measurement on polymers.</a> Langmuir. 23, 6875-6878 (2007).</li><li>Urquhart, A. J., Taylor, M., Anderson, D. G., Langer, R., Davies, M. C., Alexander, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TOF-SIMS+analysis+of+a+576+micropatterned+copolymer+array+to+reveal+surface+moieties+that+control+wettability.">TOF-SIMS analysis of a 576 micropatterned copolymer array to reveal surface moieties that control wettability.</a> Analytical Chemistry. 80, 135-142 (2008).</li><li>Hook, A. L., Yang, J., Chen, X., Roberts, C. J., Mei, Y., Anderson, D. G., Langer, R., Alexander, M. R., Davies, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acrylate+polymers+with+hydro-responsive+topography.">Acrylate polymers with hydro-responsive topography.</a> Soft Matter. 7, 7194-9197 (2011).</li><li>Pernagallo, S., Unciti-Broceta, A., Diaz-Mochon, J. J., Bradley, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deciphering+cellular+morphology+and+biocompatibility+using+polymer+microarrays.">Deciphering cellular morphology and biocompatibility using polymer microarrays.</a> Biomedical Materials. 3, (2008).</li></ol>

我々は、開示することは何もない。

ポリマーマイクロアレイは、正常な生物学的アッセイにおける新規ポリマーのスクリーニング百人の新しい材料の発見のために使用され、その後に有用なデバイスにスケールアップすることができます &#39;ヒット&#39;材料を特定されています。この場合には、記載の表面特性は、生物学的アッセイに後続を用いることができるともっぱら &#39;ヒット&#39;の材料に、より詳細にこれらの材料を研究する。 HTSCはこのアプローチを採用した実験家に利用できない場合、この戦略には興味があるかもしれません。ただし、完全に生体材料の相互作用に先立って、その後、一般的な構造と機能の動向を観察するために使用することができますHTSC​​方法論を用いて、生物学的アッセイに分析されるべきである材料の何百もの配列全体を勉強するポリマーマイクロアレイを利用する。 コンタクトプリント、ピンホルダー内で自由に上下にスライドさせて金属製のピンに依存しています。ピンとピンホルダー清潔最優先は、pを確保されているrintingが正常に行われ、厳格に行われるべきである。印刷がピンホルダー内ピンの適切な運動を実行開始前に存在するNOモノマーと、予行演習を行うことによってテストすることができます。ピンの動きが再現可能に達成されるまで、洗浄工程は継続すべきである。 かなりの思考は、モノマー混合物の設計に行く必要があります。容易に重合体のコンビナトリアルライブラリーを作製するためには、共重合体の数百の異なる比率で少数のモノマーを混合することにより形成される。これはスライドガラスの構造に適している24×24アレイを形成すると、通常、我々は576のメンバーのライブラリーを作製する。最も簡単な方法は、2:1の比率でペアワイズ24モノマーを混合することで、ほとんどの組み合わせ空間を探索コンビナトリアルライブラリーを生成するために。代わりに、アレイ内の組成勾配を含めることは、最適なモノマー組成物は、dにすることができ動向の観測を可能にするために有用であるetermined。この一例として22のモノマーは、順次6第2成分の1で希釈し、共単量体混合物の最初のコンポーネントとして使用することができます。 5希釈液を使用する場合は、例えば90:10、75:25、50:50、25:75と10:90の比率で第一及び第二の成分を混合し、これは488ユニークな共重合体ソリューションをもたらすでしょう。 576に合計を表示させるには、しばしば重要な基準試料で導入することができ、使用するモノマーのホモポリマーの複製。 576モノマー溶液は2 384ウェルプレートに分注しなければならない。ロボットをプログラミングするためにそれはモノマーの位置の観点から2つの同一のプレートを持っている方が簡単で、このようにして、モノマー溶液は、二つのプレートの間で均等に分割されるべきである。 かなりの時間がマルチチャンネルピペットを使用してソース·プレートの準備のために保存することができ、ソースプレートのデザインはマルチチャンネルピペットの使用を活用するために決定されるべきである。&lt;/ P&gt; 配列の自動化された高温超電導体を達成するために、スポットの位置が正常に特性評価装置を用いて整列する必要があります。一般的にアクリルアレイのピッチは500〜1000程度であり、ポリマーのスポット径は300μmである。ほとんどのXYステージが10μm以下の分解能を持って、このように正確な寸法は、サンプルの位置決めソフトウェアへの入力が完了したら確実に配列位置にアクセスするための表面特性評価装置のための十分な耐性があります。自動位置決めに制限は実際には配列の正確な印刷です。正確な印刷を確実にするためには、適切なスライド寸法（米国およびEUの標準のスライドサイズの両方が存在することに注意してください）​​と一緒に真空吸引またはスプリングクランプを使用して、いずれかの印刷ステージ上の基板の移動を防止することが重要である。 TOF-SIMSは、試料上の任意の汚れを観察します非常に表面敏感な手法である。このように、最大​​限の注意を払わなければなりません表面との接触を避けることができます。初めてサンプルが処理されますが、興味のある表面がきれいな手袋（好ましくはポリエチレン）を使用し、新たに洗浄ピンセットで、物と接触すべきではない。我々は通常、クロロホルム、ヘキサンで洗ってください。 5スライドホルダーまたは20スライドホルダー例えば測定前に試料の保存は、最高の、離れてスライドを保持する試料ホルダーに行われます。 配列はつまり、多くの生物学的アッセイフォーマットと読み出しと互換性があるように特別に設計され、使用される基板は、理想的には蛍光スキャナーや光顕微鏡に適した顕微鏡スライドです。これは、フォーマットは、多くの材料·生物学的相互作用を探索するために最適であることを意味します。さらに、形式は材料の数百を並列にスクリーニングすることができます。これは、より多くの材料がそれぞれの新しい材料化学を個別にスクリーニングすることにより、従来の手法に比べてスクリーニングすることができます。生物学的材料の相互作用のための増加範囲は許す生物学的な表面の相互作用だけでなく、特定のアプリケーションに対して最適な素材を見つけるのメカニズムの解明のためだ。

<table border="1"><tbody><tr><td> 試薬/機器の名称 </td><td> 会社 </td><td> カタログ/モデル番号 </td></tr><tr><td>エポキシスライド</td><td> GENETIX </td><td> K2652 </td></tr><tr><td>プリンタにお問い合わせください</td><td>バイオドット</td><td> XYZ3060プラットフォーム</td></tr><tr><td>金属製のピン</td><td> ArrayIt </td><td> 946MP6B </td></tr><tr><td> TOF-SIMSの楽器</td><td> ION-TOF </td><td></td></tr><tr><td> XPSの楽器</td><td>クラトス</td><td></td></tr><tr><td> WCAの装置</td><td>クルス</td><td> DSA 100 </td></tr><tr><td> AFM </td><td>ブルカー</td><td>ディメンション·アイコン</td></tr><tr><td> RPMI-1640細胞培養培地</td><td>シグマアルドリッチ</td><td> R0883 </td></tr><tr><td> SYTO17 </td><td>インビトロジェン</td><td> S-7579 </td></tr></tbody></table>

polymer microarrays for high throughput discovery of biomaterials

混合は均一な混合物に2つ以上のコンポーネントを組み合わせて単位操作である。この作品は、インラインスタティックミキサーを使用して2つの粘稠な液体で流れを混合伴います。ミキサーは、コンポーネント間の界面接触を高めるためにせん断および伸長流れを採用し、分割と再結合し、デザインです。プロトタイプの分割と再結合 - （SAR）のミキサーは、PVCパイプに所定の場所に保持細いレーザーカットポリメチルメタクリレート（PMMA）板のシリーズを合わせて構築した。この装置の中で混合することを図で写真に示されている。 1。赤い色素は試験流体の一部に加えて、主要な（未染色）の成分に混入する微量成分として使用した。それは混合部を流れるようにミキサーの入口において、トレーサ液の注入された層が2層に分かれています。後続の各混合部で、水平層の数が重複しています。最終的には、色素の単一ストリームは均一に分散されているTHR装置の断面図でoughout。 0.2パーセントカルボポルと類似した組成のドープトレーサー液の非ニュートン試験流体を使用して、ユニットに混合することにより、磁気共鳴イメージング（MRI）を用いて可視化されています。 MRIは、分子の化学的および物理的な環境だけでなく、ミクロンからセンチメートルに長さのスケールで試料構造の非常に強力な実験的なプローブです。この感度は、人間からの多孔質媒体1,2〜食品に至るまで材料の物理的、化学的および/ ​​または生物学的特性を把握することは、これらの技術の広範なアプリケーションをもたらしました。ここで使用される機器や条件は、普通の水と油を含む有機液体としてNMRの携帯電話、1 H、かなりの量を含むイメージング液体に適しています。伝統的MRIは、工業環境に適しており、実験室（ 図2）内に移植できませんではありません超伝導磁石を利用している。最近の進歩In個の磁石技術は、イメージングプロセス·フローに適した大容量の工業的に互換性のある磁石の建設を許可している。ここでは、MRIは混合プロセス中に異なる軸方向位置で空間分解成分濃度を提供しています。パーソナルケア製品への応用分配混合経由して高粘性流体の混合時間、リアルタイムのこの作品はドキュメント。

Watch this Scientific Journal Video about Polymer Microarrays for High Throughput Discovery of Biomaterials at JoVE.com

Polymer Microarrays for High Throughput Discovery of Biomaterials

オンチップ光重合技術を用いた高分子マイクロアレイの形成の説明。原子間力顕微鏡、水接触角の測定値を使用して高スループット表面特性、飛行二次イオン質量分析法と細胞接着アッセイのX線光電子分光法と時間も記載されている。

バイオマテリアルのハイスループット検出用高分子マイクロアレイ

The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a ...

polymer-microarrays-for-high-throughput-discovery-of-biomaterials

Research

JoVE Journal

Bioengineering

14.4K ビュー.  University of Nottingham. オンチップ光重合技術を用いた高分子マイクロアレイの形成の説明。原子間力顕微鏡、水接触角の測定値を使用して高スループット表面特性、飛行二次イオン質量分析法と細胞接着アッセイのX線光電子分光法と時間も記載されている。

ビデオ: Polymer Microarrays for High Throughput Discovery of Biomaterials

Candida albicans Biofilm Chip (CaBChip) for High-throughput Antifungal Drug Screening

Candida albicans remains the main etiological agent of candidiasis, which currently represents the fourth most common nosocomial bloodstream infection in US hospitals1. These opportunistic infections pose a growing threat for an increasing number of compromised individuals, and carry unacceptably high mortality rates. This is in part due to the limited arsenal of antifungal drugs, but also to the emergence of resistance against the most commonly used antifungal agents. Further complicating treatment is the fact that a majority of manifestations of candidiasis are associated with the formation of biofilms, and cells within these biofilms show increased levels of resistance to most clinically-used antifungal agents2. Here we describe the development of a high-density microarray that consists of C. albicans nano-biofilms, which we have named CaBChip3. Briefly, a robotic microarrayer is used to print yeast cells of C. albicans onto a solid substrate. During printing, the yeast cells are enclosed in a three dimensional matrix using a volume as low as 50 nL and immobilized on a glass substrate with a suitable coating. After initial printing, the slides are incubated at 37 &deg;C for 24 hours to allow for biofilm development. During this period the spots grow into fully developed "nano-biofilms" that display typical structural and phenotypic characteristics associated with mature C. albicans biofilms (i.e. morphological complexity, three dimensional architecture and drug resistance)4. Overall, the CaBChip is composed of ~750 equivalent and spatially distinct biofilms; with the additional advantage that multiple chips can be printed and processed simultaneously. Cell viability is estimated by measuring the fluorescent intensity of FUN1 metabolic stain using a microarray scanner. This fungal chip is ideally suited for use in true high-throughput screening for antifungal drug discovery. Compared to current standards (i.e. the 96-well microtiter plate model of biofilm formation5), the main advantages of the fungal biofilm chip are automation, miniaturization, savings in amount and cost of reagents and analyses time, as well as the elimination of labor intensive steps. We believe that such chip will significantly speed up the antifungal drug discovery process.

Candida albicans Biofilm Chip CaBChip for High-throughput Antifungal Drug Screening

We have developed a high-density microarray platform consisting of 3D nano-biofilms of C. albicans called CaBChip. The susceptibility profile of drugs tested on a CaBChip is comparable to the conventional 96-well plate model, suggesting that the fungal chip is ideally suited for true high-throughput screening of antifungal drugs.

ハイスループット抗真菌薬スクリーニングのためのカンジダバイオチップ（ カルシウム BChip）

Candida  albicans remains the main etiological agent of candidiasis, which  currently represents the fourth most common nosocomial bloodstream infection ...

生物工学

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Polyanhydrides are a class of biomaterials with excellent biocompatibility and drug delivery capabilities. While they have been studied extensively with conventional one-sample-at-a-time synthesis techniques, a more recent high-throughput approach has been developed enabling the synthesis and testing of large libraries of polyanhydrides1. This will facilitate more efficient optimization and design process of these biomaterials for drug and vaccine delivery applications. The method in this work describes the combinatorial synthesis of biodegradable polyanhydride film and nanoparticle libraries and the high-throughput detection of protein release from these libraries. In this robotically operated method (Figure 1), linear actuators and syringe pumps are controlled by LabVIEW, which enables a hands-free automated protocol, eliminating user error. Furthermore, this method enables the rapid fabrication of micro-scale polymer libraries, reducing the batch size while resulting in the creation of multivariant polymer systems. This combinatorial approach to polymer synthesis facilitates the synthesis of up to 15 different polymers in an equivalent amount of time it would take to synthesize one polymer conventionally. In addition, the combinatorial polymer library can be fabricated into blank or protein-loaded geometries including films or nanoparticles upon dissolution of the polymer library in a solvent and precipitation into a non-solvent (for nanoparticles) or by vacuum drying (for films). Upon loading a fluorochrome-conjugated protein into the polymer libraries, protein release kinetics can be assessed at high-throughput using a fluorescence-based detection method (Figures 2 and 3) as described previously1. This combinatorial platform has been validated with conventional methods2 and the polyanhydride film and nanoparticle libraries have been characterized with 1H NMR and FTIR. The libraries have been screened for protein release kinetics, stability and antigenicity; in vitro cellular toxicity, cytokine production, surface marker expression, adhesion, proliferation and differentiation; and in vivo biodistribution and mucoadhesion1-11. The combinatorial method developed herein enables high-throughput polymer synthesis and fabrication of protein-loaded nanoparticle and film libraries, which can, in turn, be screened in vitro and in vivo for optimization of biomaterial performance.

This method describes the combinatorial synthesis of biodegradable polyanhydride film and nanoparticle libraries and the high-throughput detection of protein release from these libraries.

組合せの合成と高分子膜とナノ粒子のライブラリからのハイスループットタンパクリリース

Polyanhydrides are a class of biomaterials with excellent biocompatibility and drug delivery capabilities. While they have been studied extensively with ...

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Transdisciplinary approaches involving areas such as material design, nanotechnology, chemistry, and immunology have to be utilized to rationally design efficacious vaccines carriers. Nanoparticle-based platforms can prolong the persistence of vaccine antigens, which could improve vaccine immunogenicity1. Several biodegradable polymers have been studied as vaccine delivery vehicles1; in particular, polyanhydride particles have demonstrated the ability to provide sustained release of stable protein antigens and to activate antigen presenting cells and modulate immune responses2-12.
The molecular design of these vaccine carriers needs to integrate the rational selection of polymer properties as well as the incorporation of appropriate targeting agents. High throughput automated fabrication of targeting ligands and functionalized particles is a powerful tool that will enhance the ability to study a wide range of properties and will lead to the design of reproducible vaccine delivery devices.
The addition of targeting ligands capable of being recognized by specific receptors on immune cells has been shown to modulate and tailor immune responses10,11,13 C-type lectin receptors (CLRs) are pattern recognition receptors (PRRs) that recognize carbohydrates present on the surface of pathogens. The stimulation of immune cells via CLRs allows for enhanced internalization of antigen and subsequent presentation for further T cell activation14,15. Therefore, carbohydrate molecules play an important role in the study of immune responses; however, the use of these biomolecules often suffers from the lack of availability of structurally well-defined and pure carbohydrates. An automation platform based on iterative solution-phase reactions can enable rapid and controlled synthesis of these synthetically challenging molecules using significantly lower building block quantities than traditional solid-phase methods16,17.
Herein we report a protocol for the automated solution-phase synthesis of oligosaccharides such as mannose-based targeting ligands with fluorous solid-phase extraction for intermediate purification. After development of automated methods to make the carbohydrate-based targeting agent, we describe methods for their attachment on the surface of polyanhydride nanoparticles employing an automated robotic set up operated by LabVIEW as previously described10. Surface functionalization with carbohydrates has shown efficacy in targeting CLRs10,11 and increasing the throughput of the fabrication method to unearth the complexities associated with a multi-parametric system will be of great value (Figure 1a).

In this article, a high throughput method is presented for the synthesis of oligosaccharides and their attachment to the surface of polyanhydride nanoparticles for further use in targeting specific receptors on antigen presenting cells.

無水物ナノ粒子のハイスループット糖鎖の合成と機能化

Transdisciplinary approaches involving areas such as material design, nanotechnology, chemistry, and immunology have to be utilized to rationally design ...

Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models

Renal function and continence of urine are critically dependent on the proper function of the urinary bladder, which stores urine at low pressure and expels it with a precisely orchestrated contraction. A number of congenital and acquired urological anomalies including posterior urethral valves, benign prostatic hyperplasia, and neurogenic bladder secondary to spina bifida/spinal cord injury can result in pathologic tissue remodeling leading to impaired compliance and reduced capacity1. Functional or anatomical obstruction of the urinary tract is frequently associated with these conditions, and can lead to urinary incontinence and kidney damage from increased storage and voiding pressures2. Surgical implantation of gastrointestinal segments to expand organ capacity and reduce intravesical pressures represents the primary surgical treatment option for these disorders when medical management fails3. However, this approach is hampered by the limitation of available donor tissue, and is associated with significant complications including chronic urinary tract infection, metabolic perturbation, urinary stone formation, and secondary malignancy4,5.
Current research in bladder tissue engineering is heavily focused on identifying biomaterial configurations which can support regeneration of tissues at defect sites. Conventional 3-D scaffolds derived from natural and synthetic polymers such as small intestinal submucosa and poly-glycolic acid have shown some short-term success in supporting urothelial and smooth muscle regeneration as well as facilitating increased organ storage capacity in both animal models and in the clinic6,7. However, deficiencies in scaffold mechanical integrity and biocompatibility often result in deleterious fibrosis8, graft contracture9, and calcification10, thus increasing the risk of implant failure and need for secondary surgical procedures. In addition, restoration of normal voiding characteristics utilizing standard biomaterial constructs for augmentation cystoplasty has yet to be achieved, and therefore research and development of novel matrices which can fulfill this role is needed.
In order to successfully develop and evaluate optimal biomaterials for clinical bladder augmentation, efficacy research must first be performed in standardized animal models using detailed surgical methods and functional outcome assessments. We have previously reported the use of a bladder augmentation model in mice to determine the potential of silk fibroin-based scaffolds to mediate tissue regeneration and functional voiding characteristics.11,12 Cystometric analyses of this model have shown that variations in structural and mechanical implant properties can influence the resulting urodynamic features of the tissue engineered bladders11,12. Positive correlations between the degree of matrix-mediated tissue regeneration determined histologically and functional compliance and capacity evaluated by cystometry were demonstrated in this model11,12. These results therefore suggest that functional evaluations of biomaterial configurations in rodent bladder augmentation systems may be a useful format for assessing scaffold properties and establishing in vivo feasibility prior to large animal studies and clinical deployment. In the current study, we will present various surgical stages of bladder augmentation in both mice and rats using silk scaffolds and demonstrate techniques for awake and anesthetized cystometry.

Surgical stages of bladder augmentation are described using 3-D scaffolds in murine and rat models. To test the efficacy of biomaterial configurations for use in bladder augmentation, techniques for both awake and anesthetized cystometry are presented.

各種齧歯類モデルで内圧分析を用いた膀胱補強のためのバイオマテリアルの評価

Renal function and continence of urine are critically dependent on the proper function of the urinary bladder, which stores urine at low pressure and ...

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to evaluate new strategies that aim at improving vascularization, reliable methods are required to make the in-growth of new blood vessels into bio-artificial scaffolds visible and quantify the results. Over the past couple of years, our group has introduced a full skin defect model that enables the direct visualization of blood vessels by transillumination and provides the possibility of quantification through digital segmentation. In this model, one surgically creates full skin defects in the back of mice and replaces them with the material tested. Molecules or cells of interest can also be incorporated in such materials to study their potential effect. After an observation time of one&#8217;s own choice, materials are explanted for evaluation. Bilateral wounds provide the possibility of making internal comparisons that minimize artifacts among individuals as well as of decreasing the number of animals needed for the study. In comparison to other approaches, our method offers a simple, reliable and cost effective analysis. We have implemented this model as a routine tool to perform high-resolution screening when testing vascularization of different biomaterials and bio-activation approaches.

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.

生体材料の血管新生を評価するために、完全な皮膚欠損モデル<em&gt;インビボ</em

Insufficient vascularization is considered to be one of the main factors limiting the clinical success of tissue-engineered constructs. In order to ...

A Coupled Experiment-finite Element Modeling Methodology for Assessing High Strain Rate Mechanical Response of Soft Biomaterials

This study offers a combined experimental and finite element (FE) simulation approach for examining the mechanical behavior of soft biomaterials (e.g. brain, liver, tendon, fat, etc.) when exposed to high strain rates. This study utilized a Split-Hopkinson Pressure Bar (SHPB) to generate strain rates of 100-1,500 sec-1. The SHPB employed a striker bar consisting of a viscoelastic material (polycarbonate). A sample of the biomaterial was obtained shortly postmortem and prepared for SHPB testing. The specimen was interposed between the incident and transmitted bars, and the pneumatic components of the SHPB were activated to drive the striker bar toward the incident bar. The resulting impact generated a compressive stress wave (i.e. incident wave) that traveled through the incident bar. When the compressive stress wave reached the end of the incident bar, a portion continued forward through the sample and transmitted bar (i.e. transmitted wave) while another portion reversed through the incident bar as a tensile wave (i.e. reflected wave). These waves were measured using strain gages mounted on the incident and transmitted bars. The true stress-strain behavior of the sample was determined from equations based on wave propagation and dynamic force equilibrium. The experimental stress-strain response was three dimensional in nature because the specimen bulged. As such, the hydrostatic stress (first invariant) was used to generate the stress-strain response. In order to extract the uniaxial (one-dimensional) mechanical response of the tissue, an iterative coupled optimization was performed using experimental results and Finite Element Analysis (FEA), which contained an Internal State Variable (ISV) material model used for the tissue. The ISV material model used in the FE simulations of the experimental setup was iteratively calibrated (i.e. optimized) to the experimental data such that the experiment and FEA strain gage values and first invariant of stresses were in good agreement.

The current study prescribes a coupled experiment-finite element simulation methodology to obtain the uniaxial dynamic mechanical response of soft biomaterials (brain, liver, tendon, fat, etc.). The multiaxial experimental results that arose because of specimen bulging obtained from Split-Hopkinson Pressure Bar testing were rendered to a uniaxial true stress-strain behavior when simulated through iterative optimization of the finite element analysis of the biomaterial.

ソフトバイオマテリアルの高ひずみ速度機械的応答を評価するための結合実験、有限要素モデリング手法

This study offers a combined experimental and finite element (FE) simulation approach for examining the mechanical behavior of soft biomaterials (e.g. ...

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Cryo-electron tomography (Cryo-ET) is a powerful three-dimensional (3-D) imaging technique for visualizing macromolecular complexes in their native context at a molecular level. The technique involves initially preserving the sample in its native state by rapidly freezing the specimen in vitreous ice, then collecting a series of micrographs from different angles at high magnification, and finally computationally reconstructing a 3-D density map. The frozen-hydrated specimen is extremely sensitive to the electron beam and so micrographs are collected at very low electron doses to limit the radiation damage. As a result, the raw cryo-tomogram has a very low signal to noise ratio characterized by an intrinsically noisy image. To better visualize subjects of interest, conventional imaging analysis and sub-tomogram averaging in which sub-tomograms of the subject are extracted from the initial tomogram and aligned and averaged are utilized to improve both contrast and resolution. Large datasets of tilt-series are essential to understanding and resolving the complexes at different states, conditions, or mutations as well as obtaining a large enough collection of sub-tomograms for averaging and classification. Collecting and processing this data can be a major obstacle preventing further analysis. Here we describe a high-throughput cryo-ET protocol based on a computer-controlled 300kV cryo-electron microscope, a direct detection device (DDD) camera and a highly effective, semi-automated image-processing pipeline software wrapper library tomoauto developed in-house. This protocol has been effectively utilized to visualize the intact type III secretion system (T3SS) in Shigella flexneri minicells. It can be applicable to any project suitable for cryo-ET.

We present a protocol on how to utilize high-throughput cryo-electron tomography to determine high resolution in situ structures of molecular machines. The protocol permits large amounts of data to be processed, avoids common bottlenecks and reduces resource downtime, allowing the user to focus on important biological questions.

Tomoautoの使用：ハイスループット自動クライオ電子断層撮影のためのプロトコル

Cryo-electron tomography (Cryo-ET) is a powerful three-dimensional (3-D) imaging technique for visualizing macromolecular complexes in their native ...

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Studying the heterogeneity of single cells is crucial for many biological questions, but is technically difficult. Thus, there is a need for a simple, yet high-throughput, method to perform single-cell culture experiments. Here, we report a microfluidic chip-based strategy for high-efficiency single-cell isolation (~77%) and demonstrate its capability of performing long-term single-cell culture (up to 7 d) and cellular heterogeneity analysis using clonogenic assay. These applications were demonstrated with KT98 mouse neural stem cells, and A549 and MDA-MB-435 human cancer cells. High single-cell isolation efficiency and long-term culture capability are achieved by using different sizes of microwells on the top and bottom of the microfluidic channel. The small microwell array is designed for precisely isolating single-cells, and the large microwell array is used for single-cell clonal culture in the microfluidic chip. This microfluidic platform constitutes an attractive approach for single-cell culture applications, due to its flexibility of adjustable cell culture spaces for different culture strategies, without decreasing isolation efficiency.

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

ハイスループットシングルセルの単離および培養のためのマイクロ流体プラットフォーム

Studying the heterogeneity of single cells is crucial for many biological questions, but is technically difficult. Thus, there is a need for a simple, yet ...

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Medical devices are often associated with hospital-acquired infections, which place enormous strain on patients and the healthcare system as well as contributing to antimicrobial resistance. One possible avenue for the reduction of device-associated infections is the identification of bacteria-repellent polymer coatings for these devices, which would prevent bacterial binding at the initial attachment step. A method for the identification of such repellent polymers, based on the parallel screening of hundreds of polymers using a microarray, is described here. This high-throughput method resulted in the identification of a range of promising polymers that resisted binding of various clinically relevant bacterial species individually and also as multi-species communities. One polymer, PA13 (poly(methylmethacrylate-co-dimethylacrylamide)), demonstrated significant reduction in attachment of a number of hospital isolates when coated onto two commercially available central venous catheters. The method described could be applied to identify polymers for a wide range of applications in which modification of bacterial attachment is important.

A high-throughput microarray method for the identification of polymers which reduce bacterial surface binding on medical devices is described.

医療機器のための細菌撥ポリマーのハイスループット同定

Medical devices are often associated with hospital-acquired infections, which place enormous strain on patients and the healthcare system as well as ...

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

To upgrade traditional 2D cell culture to 3D cell culture, we have integrated microfabrication with cryogelation technology to produce macroporous microscale cryogels (microcryogels), which can be loaded with a variety of cell types to form 3D microtissues. Herein, we present the protocol to fabricate versatile 3D microtissues and their applications in regenerative therapy and drug screening. Size and shape-controllable microcryogels can be fabricated on an array chip, which can be harvested off-chip as individual cell-loaded carriers for injectable regenerative therapy or be further assembled on-chip into 3D microtissue arrays for high-throughput drug screening. Due to the high elastic nature of these microscale cryogels, the 3D microtissues exhibit great injectability for minimally invasive cell therapy by protecting cells from mechanical shear force during injection. This ensures enhanced cell survival and therapeutic effect in the mouse limb ischemia model. Meanwhile, assembly of 3D microtissue arrays in a standard 384-multi-well format facilitates the use of common laboratory facilities and equipment, enabling high-throughput drug screening on this versatile 3D cell culture platform.

This protocol describes the fabrication of elastic 3D macroporous microcryogels by integrating microfabrication with cryogelation technology. Upon loading with cells, 3D microtissues are generated, which can be readily injected in vivo to facilitate regenerative therapy or assembled into arrays for in vitro high-throughput drug screening.