National Institute on Drug Abuse, NIH grant 5R44DA026220-03

Here the use of the OPAD (Stoelting Co., Wood Dale, IL) is described in general terms for an example experiment using capsaicin. The operator has the freedom to program numerous experiments with many options and pain models though. For instance, the administration of analgesics reduce nociceptive measures 6,3,4,5,7 and other pain models like chronic constriction injury produce and inflammation increased nociceptive behaviors 3,9. These models are easily adaptable to the following protocol.
For all experiments, maleSprague-Dawley rats (250-300 g, Charles River, Raleigh, NC) were used. These were housed in pairs in 22 &deg;C temperature and 31% humidity controlled rooms with a normal 12-hr light/dark cycles (6am-6pm lights on) and had free access to food and water except when fasted. Behavioral sessions were performed during the light phase. These facilities were AAALAC accredited and all procedures were approved by the University of Florida IACUC.
1.Training and Baseline Sessions
<ol>
 <li>Food fast rodents the night before each OPAD session (ex. 15+/- 1 hr for this experiment).</li>
 <li>Rodents must first be trained until consistent behavior at non-aversive temperatures (ex. 33-37 &deg;C) is observed. Generally, about six sessions (three times a week for two weeks) is sufficient to train mice or rats to lick about 600-1,000 or 2,000 times per session, respectively.</li>
</ol>
2. Pretest Preparation and Capsaicin Treatment
<ol>
 <li>Hairless rodents are best for all operant procedures, if this is not possible, rodents must have their facial hair (buccal hair only and not vibrissae pad/whiskers as this has an effect on a rodent's navigation) removed 1-2 days before testing for accurate recording of the behavior.</li>
 <li>Buccal hair acts as a temperature insulator and makes heat and cold less nociceptive. To remove hair, anesthetize rats, add eye ointment, shave cheek hair with clippers, apply hair removal cream, wait 2-4 min, then wash with water. See Neubert et al. 3 for full methodology.</li>
 <li>On the day of testing, anesthetize the animal (ex. 1-2.5% isoflurane inhalation) and place opthalamic vet ointment on both eyes to prevent them from drying out and to keep any topical drug treatments from getting in the eye.</li>
 <li>Apply capsaicin cream (0.1%) bilaterally on the exposed cheeks with a sterile cotton swab. Wait 5 min. Wipe off cream with gauze pads soaked in warm water (at about 40 &deg;C). Wipe cheeks off with an alcohol swab and set a timer for 30 min.</li>
 <li>Allow the rodent sufficient time to recover from anesthesia before returning it to the cage. Wait until it can raise its head in a sternal posture in order to prevent the aspiration of cage bedding.</li>
</ol>
3. Programming the OPAD System for Protocols and Experiments
<ol>
 <li>The key innovation of the OPAD system to behavioral testing is the OroFacial software, an ANY-maze (Stoelting Co., Wood Dale, IL) driven system that allows the user to program and create new experiments.</li>
 <li>A general example of how to program a simple experiment is provided below, but many more options for temperature ramping protocols and pain models other than capsaicin are available.</li>
 <li>Experiments can be designed and saved at any point. Turn on OPADs and open software. Turn on white noise to control for ambient noise. Under "File" select New Experiment. Under the subheading "Protocol" name the protocol and select whether to be blinded.</li>
 <li>Under "OPAD cages" select "New OPAD cage" then "Add all connected OPAD cages." Under "Outputs" select "Temperature Controllers" then "Thermal element". Adjust starting temperature (for example, a neutral 33-37 &deg;C).</li>
 <li>If needed, adjust ramping temperatures under "OPAD temperature cycle." In order to change the temperature from neutral to hot to cold make adjustments here.</li>
 <li>For "1", "Set temperature to (&deg;C)" = 45, "Ramp duration" = 30 sec, and "Remain at value for" = 3 min. For "2", 33, 30 sec, and 3 min. For "3", 7, 60 sec, and 3 min. For "4", 33, 60 sec, and 3 min. Check the box for "After the following period of time:" and select 3 min.</li>
 <li>Under "Fields" add any extra notes about the subjects. For example, make an area for animal ID by selecting "New Field" and change the name to "Animal ID." Then choose "Animals", "Text", and "Use this field as the animal ID."</li>
 <li>Under "Stage", the OPAD will automatically set a "First stage." Set duration of test period and name the stage if needed. For this ramping session, set this to 18 min.</li>
 <li>A note on stages: for most experiments, behavioral sessions shouldn't last for longer than 10-20 min. After that, rodents are satiated. For experiments over several days, additional stages can be added for each day to make the data analysis simpler.</li>
 <li>Under "Calculations" select "New Calculation" and name it "L/F" for lick/face ratio. In the section labeled "Enter the calculation in the area below" adjust to say "Lick : number of activations / Contact : number of activations".</li>
 <li>To create time periods for a simpler data analysis select "Analysis" and "New time period". Name one "33 &deg;C" and select the box for "This period is the same in all stages" For "Starts at:" write 0 and for "Ends at:" put 3 min.</li>
 <li>Repeat the above step for each time period. Name: "Ramp 33-45 &deg;C." Starts at: 3 min. Ends at: 3.5 min. "45 &deg;C", 3.5 min, 6.5 min. "Ramp 45-33 &deg;C", 6.5 min, 7 min. "33 &deg;C", 7 min, 10 min. "Ramp 33-7 &deg;C", 10 min, 11 min. "7 &deg;C", 11 min, 14 min. "Ramp 7-33 &deg;C", 14 min, 15 min. "33 &deg;C", 15 min, 18 min.</li>
 <li>Save and name the protocol. Note: saved protocols can be re-used in new experiments.</li>
</ol>
4. Running the Assay
<ol>
 <li>Prepare a room temperature reward mixture and place in OPAD bottles. A 2:1 ratio of water:sweetened condensed milk works well although sucrose or saccharin solutions may also be used.</li>
 <li>Place liquid catch tray, Plexiglas cage, and metal flooring grate on the OPAD machine. Attach wiring to cage.</li>
 <li>Place reward bottle on stand and adjust so the spout can be reached by the rodent. Initially, the bottle can be placed further back in the cage then withdrawn to produce better facial contacts.</li>
 <li>Load the experiment into the OPAD software. Add the number of animals to be tested. Under the subheading "Experiment" add a "Title" and then add the treatment groups (capsaicin and control). Add the number of animals in each group.</li>
 <li>Under the subheading "Tests", select "Animal treatments and data". Add the letter of the treatment (A, B, C, etc.) and the animal ID. The boxes should now have the designated animal ID on their screens.</li>
 <li>Press the button on the OPAD box. This will adjust the thermodes to the right temperature. When the light goes orange, place the rodent inside and press the button again. For this experiment, rats will be started 30 min after the capsaicin cream was wiped off.</li>
 <li>The green light will turn on. Adjust the distance the bottle is from the box so the rat must make contact with the thermodes on its buccal, not vibrissa, region to be able to lick. Proper adjustment will result in a solid red box for licking above an outlined orange box for contacts.</li>
 <li>Proper placement of the reward bottle is vital for the assay. If the bottle is too close the rat will either lick without making a contact or to touch its nose to the reward bottle so that many licks will appear as only one (denoted by an outlined red box for licks instead of a solid box).</li>
 <li>Once the testing session is over the OPAD will alert the experimenter with a tone. Return the rodent to its cage. If another rodent is to be tested afterward the box will indicate its Animal ID. Repeat steps 4.5-4.7 as needed until the experiment is complete.</li>
</ol>
5. Analyzing, Graphing, and Statistical Analysis of Data with OPAD Software
<ol>
 <li>Under the subheading "Results" select whether to see a text, graph, or statistical analysis report.</li>
 <li>In the "Graph report settings" box, select which variable to examine. For instance, under "Calculation results" check the "L/F" box.</li>
 <li>Specify "On the x-axis show" as "Time period" and "Show different series for" as "Treatment." Select "View the report". Saving, printing, copying, or emailing the report can be done at this time. Certain data points may be excluded in the box below the grouping step if needed.</li>
 <li>Under the subheading "Data" is an adjustable listing of the raw data from the experiment in spreadsheet form if needed. Note: all the data is now saved and can be manipulated and analyzed at a later time.</li>
</ol>
6. Clean Up
<ol>
 <li>Shut off the machine and remove cage wiring. Wash and sanitize the grate, box, bottle, and liquid tray. These components can be washed by hand or dishwasher.</li>
</ol>

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Richard Mills and Chris Lloyd are employees of Stoelting Co.
Production and Free Access to this article is sponsored by Stoelting Co.

The OPAD system is an easy to use, high throughput assay capable of detecting changes in pain perception in rodents. The high throughput nature of this system means that numerous animals can be tested in a single day by a single person. This is due to the OPAD software system as it allows up to 16 boxes to be run concurrently on a single computer. This means that after the initial setup time, about 48 operant runs (at 18 min per run) can be performed an hour, even more if the session time is set to less time per stage. This allows for pain testing in hundreds of animals a day. This amount of testing would not be practical with most traditional pain assays.
Consistent with our previous work, rodent behavior is altered under painful conditions. During the non-noxious periods rodents typically have long bouts of drinking in which they maintain contact with the thermodes. During aversive 45 &deg;C or 7 &deg;C conditions, the rodents have much shorter bouts as they cannot maintain contact for long periods of time. Therefore the lick/face ratio (number of licks divided by the number of facial contacts within a session) alters with pain. Capsaicin increased the sensitivity to heat pain as demonstrated by a lower L/F ratio in the treated versus untreated rodents at the 45 &deg;C temperature. Analgesics can return this lick/face ratio to levels similar to non-painful conditions 3. Although pain conditions that are easily produced on skin (like the application of capsaicin cream) are the simplest methods of detecting pain on this assay, animal models of more clinically relevant deep neural tissue pain like trigeminal neuralgia can also alter behavior on operant orofacial assays 9. Taken together these data are supporting evidence that the OPAD is sensitive to alterations in heat and cold pain, pain thresholds, and noxious chemical agents like capsaicin in addition to the operant orofacial pain assay's ability to detect numerous other conditions of pain and analgesia 6,3,8,17,10,5,12,18,11,9. 
The OPAD's system of measuring pain is a more clinically relevant, meaningful, and humane method of detecting pain than reflex-based measures. These traditional measures of nociception like the paw withdrawal with von Frey filaments 19 and the tail-flick assay 20 have been used for over a century but they only measure the response to an experimenter-inflicted stimulus. The animal has little control and the "nociception" is mainly localized to the spinal cord. For humans, the subjective pain experience is also important as people are simply asked to report their subjective levels of pain. The ability for animals to self-report their pain in operant based procedures would be a breakthrough for basic pain research 1. With the OPAD, animals are given the choice of whether to respond during a painful stimulus or not. If it is too painful, animals simply reduce their attempts to reach the reward and thus limit their exposure to pain. This is a much more humane and less stressful assay when compared to many reflex-based measures in which animals often have their movement restricted and have no control over the amount of painful stimuli to which they are exposed. The need to escape from pain is an inherent drive in all animals and the OPAD incorporates this behavior instead of compensating for it like other nociceptive assays. The movement away from reflex-based measures of pain into operant tasks is becoming more common in the field. Other groups have used non-reflex-based measures like examining meal duration 21,22,23 and thermal heat pain escape paradigms 24 (For a review of other pain measures we suggest our first reference 1). The OPAD is able to combine elements of these into a unified measure, the Lick/Face ratio, which examines food intake and the need to escape from painful stimuli. Another benefit is that this assay is capable of measuring pain over long periods of time (1-2 months) without losing sensitivity 7,9. Due to its advantages over reflex-based testing, this less stressful and more humane assay is well adapted to measuring long-term changes in nociceptive behavior in rodents.
Operant pain measures often give different results when compared to reflex-based measures in terms of opioid dose effects and pain thresholds. While high doses of opioids are typically used for reflex-based measures 25 several studies indicate that lower doses are needed for responses on operant assays 26,27,28. High drug doses could also interfere with operant measures but these are detectable with the OPAD 6. Other studies have also demonstrated that the thresholds for escape from a painful stimulus are different for operant versus reflex-based measures 29,2,30 suggesting a major difference between an animals' perception of pain versus the speed of their spinal reflexes. A benefit of the OPAD is that the rodent can choose whether or not to perform the task, this allows the rodent to express escape or avoidance behavior. This complex behavior requires cortical decision making to control the amount of nociception the rodent feels 14,29,15,30. While escape and avoidance behaviors can interfere with reflex based measures these pain behaviors are an integral component of the OPAD. The differences in pain thresholds and the lower doses of opioids needed for operant assays suggest a higher sensitivity to pain and analgesia than traditional reflex-based measures.
Although the OPAD can measure pain more directly than traditional assays, several experimental conditions and drugs could have an adverse effect on this assay and must be controlled for. Alterations in appetitive motivation can alter behavior on this assay. This could be reflected by a difference in the reward itself 31 or by the motivation for the reward 6. Care must be taken to ensure that the animal's motivation for the reward is constant as many drugs can interfere with motivation. For instance, high doses of morphine and other opioids can cause hyperphagia for sweet, fatty substances 32 which will alter responding on the operant orofacial assay 6. While this does suggest that this operant-based reward-conflict paradigm has wider implications for use including the fields of anxiety and addiction (i.e. changing the rewarding aspects in the presence of a given painful stimulus) it is important to control for appetitive alterations during pain testing sessions. These alterations in motivation do not appear at these clinically relevant lower doses but the analgesic effects remain intact 3. One way to control for this possible confound is to ensure the dose of the drug given does not increase behavior at a neutral (33-37 &deg;C) temperature. Testing a drug versus a non-drug group at a neutral temperature should be a first step before adding a pain component. Also, given that several baseline sessions are possible within a test session using the OPAD these issues can be detected and can be controlled for within a single behavioral session. As the fasting schedule can alter motivation on this assay it is important to keep this consistent. We typically do an overnight fast, but other schedules are possible. For instance, we have experimented with a daily short fast of 6 hr before (unpublished results). This allows for testing daily instead of every other day. Also, unfasted rats have also responded well on the assay 9. Whatever fasting techniques are used it is mainly important to keep it consistent throughout testing to control for motivational factors.
In conclusion, the OPAD is an easy to use operant assay which measures pain on a level much more similar to the human condition than traditional pain assays. The key feature of this system is integration of the experimental parameters and protocols, data acquisition, and analysis/outcome measures using a software-controlled system. This will provide a wealth of user-controlled options and parameters to collect and analyze numerous outcome measures in a high-throughput fashion. This contrasts the commonly used pain-testing systems (e.g. tail flick, von Frey filaments) which are neither software-driven nor high-throughput. The software-driven system provides a significant advancement for how behavioral studies are designed and the how the data is collected and analyzed and an increase in the use of this assay will allow basic pain research to become more clinically translatable in the future. This system is expected to have a significant impact on advancing future research related to pain because these operant behavioral studies can provide the necessary link for understanding the influence of higher order structures on overall pain behavior.

Chronic, uncontrolled pain remains a major public health problem and novel analgesic treatments often fail to translate from the bench to bedside. This lack of success is partly due to the use of inefficient behavioral assays in reflex based measures of pain which do not necessarily or completely model the human pain condition 1,2, and specifically, a lack of a reliable, high-throughput, commercially available, in vivo pain-assessment assay for both rats and mice. We present here a high throughput, easy to use version of our operant based nociception assay. This new system is based on our previous operant orofacial pain assay which has been demonstrated to be sensitive to detecting different pain modalities including heat, cold, and mechanical 3,4,5. From these measures, a wide variety of fields have been studied, including analgesics 6,3,4,5,7, pain conditions like inflammation, hyperalgesia, and allodynia 3,8, trigeminal neuralgia 9, and peripheral nociceptive modulation via TRP channels with capsaicin, resiniferatoxin, menthol, and icillin 8,10,5,11. Psychological effects like anxiety-induced modulation of pain 12 and the placebo effect 13have also been demonstrated with orofacial operant testing suggesting it may be appropriate for measuring the full experience of pain and not simply nociception.
The Orofacial Pain Assessment Device (OPAD) uses a reward/conflict assay which allows a rodent to choose between receiving a reinforcing reward or escaping an aversive stimulus thus controlling the amount of pain it feels during a session 14,15. Rodents are first trained to press their faces into temperature controlled thermodes in order to gain access to a food bottle containing a liquid reward. After training, the stimulus temperature can be heated or cooled and differences in responding can indicate the level of nociception or analgesia the animal perceives. The OPAD is also capable of rapid changes in temperature which allows baseline testing and the assessment of pain at hot and cold temperatures within a single testing session. Here we present a simple protocol which highlights the OPAD's ability to detect changes in pain caused by heat, cold, and the TRPV1 agonist capsaicin 16. Capsaicin is used below as a thermal sensitizing agent because it has several benefits to this assay as it is non-tissue damaging and has been demonstrated previously to induce facial allodynia and hyperalgesia in rodent models 8. We will demonstrate how the OPAD software can rapidly obtain, analyze, graph, and perform statistical analyses on rodent behavioral data.

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use of the operant orofacial pain assessment device (opad) to measure changes in nociceptive behavior

We present an operant system for the detection of pain in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) assesses pain behaviors in a more clinically relevant way by not relying on reflex-based measures of nociception. Food fasted, hairless (or shaved) rodents are placed into a Plexiglas chamber which has two Peltier-based thermodes that can be programmed to any temperature between 7 &deg;C and 60 &deg;C. The rodent is trained to make contact with these in order to access a reward bottle. During a session, a number of behavioral pain outcomes are automatically recorded and saved. These measures include the number of reward bottle activations (licks) and facial contact stimuli (face contacts), but custom measures like the lick/face ratio (total number of licks per session/total number of contacts) can also be created. The stimulus temperature can be set to a single temperature or multiple temperatures within a session. The OPAD is a high-throughput, easy to use operant assay which will lead to better translation of pain research in the future as it includes cortical input instead of relying on spinal reflex-based nociceptive assays.

Typical results are illustrated for a single rodent's behavior on the OPAD in Figures 1A-D. The number of licks is high for every segment of the session at the neutral 33 &deg;C temperature, but low for aversive ones (45 &deg;C and 7 &deg;C) as illustrated in Figure 1A. As Figure 1B demonstrates, long bouts of contact are made at 33 &deg;C as is typical for non-nociceptive stimulus temperatures. The duration decreases and the number of contacts increase during periods where the temperatures are painful. Figure 1C is a diagram of the ramping protocol the OPAD was programmed to use for all test sessions. Figure 1D displays the total amount of reward ingested over time in grams. Similarly to the number of licks, animals prefer the neutral temperatures over the painful ones. The lick/face ratio (L/F) for the baseline session was calculated by the OPAD and is illustrated in Figure 1E. This ratio is much higher during the three non-painful 33 &deg;C sessions (20-46 licks per facial contact) than at the painful sessions of 45 &deg;C (3 licks per facial contact) and 7 &deg;C (1 lick per facial contact). A Repeated Measures One-Way ANOVA was significant (F(4,52)=6.2182, p&lt;0.001) for an effect of temperature on the L/F ratio. Bonferroni's test were significant when comparing 33 &deg;C vs. 7 &deg;C (p&lt;0.05), 45 &deg;C vs. 33 &deg;C (2) (p&lt;0.01), and 33 &deg;C (2) vs. 7 &deg;C (p&lt;0.01). N=16 for all temperatures. In Figure 1F the capsaicin treated rodents (N=8) were not significantly different from naive rats (N=8) at any of the neutral 33 &deg;C temperatures. Capsaicin treated rodents did have a significantly lower L/F ratio at 45 &deg;C (t-test, t(13)=2.9350, p=0.012). The capsaicin group had higher L/F ratios at 7 &deg;C, but this was not significant.
<img src="/files/ftp_upload/50336/50336fig1.jpg" alt="Figure 1" fo:content-width="3.5in" fo:src="/files/ftp_upload/50336/50336fig1highres.jpg"/> 
 Figure 1. Measuring nociception with the OPAD. A single rodent's behavior on the OPAD is graphed for A) number of licks, B) contacts, C) temperature of the thermode during the session, and D) reward intake in grams. E) The lick/face ratio is high during the three non-painful 33 &deg;C sessions and is significantly lower at the painful sessions of 45 &deg;C and 7 &deg;C (Repeated Measures One-Way ANOVA, F(4,52)=6.2182, p&lt;0.001, Bonferroni's test 33 &deg;C vs. 7 &deg;C (p&lt;0.05, #), 45 &deg;C vs. 33 &deg;C (2) (p&lt;0.01, **), and 33 &deg;C (2) vs. 7 &deg;C (p&lt;0.01, ##). F) Capsaicin treated rodents had a significantly lower L/F ratio at 45 &deg;C (t-test, t(13)=2.9350, p=0.012, *) but at none of the neutral temperatures. N=16 for 1E and N=8 for capsaicin and N=8 for naive for 1F. <a href="https://www.jove.com/files/ftp_upload/50336/50336fig1large.jpg" target="_blank"> Click here to view larger figure</a>.

Watch this Scientific Journal Video about Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior at JoVE.com

Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior

We present a user-friendly, high-throughput operant system for the evaluation of pain behaviors in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) can assess pain through a reward/conflict paradigm thus providing a more humane way of testing. This protocol will yield more clinically relevant and translational data from rodents.

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

We present an operant  system for the detection of pain in awake, conscious rodents. The Orofacial  Pain Assessment Device (OPAD) assesses pain behaviors ...

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Behavior

20.9K ビュー.  University of Florida College of Dentistry. 我々は目を覚まし、意識げっ歯の痛み行動の評価のためのユーザーフレンドリーな、高スループットオペラントシステムを提示する。口腔顔面疼痛評価デバイス（OPAD）はテストのより人道的な方法を提供するため、報酬/紛争パラダイムを通して痛みを評価することができます。このプロトコルは、げっ歯類から、より臨床的に関連すると翻訳データが得...

ビデオ: Use of the Operant Orofacial Pain Assessment Device OPAD to Measure Changes in Nociceptive Behavior

The Crossmodal Congruency Task as a Means to Obtain an Objective Behavioral Measure in the Rubber Hand Illusion Paradigm

The rubber hand illusion (RHI) is a popular experimental paradigm. Participants view touch on an artificial rubber hand while the participants' own hidden hand is touched. If the viewed and felt touches are given at the same time then this is sufficient to induce the compelling experience that the rubber hand is one's own hand. The RHI can be used to investigate exactly how the brain constructs distinct body representations for one's own body. Such representations are crucial for successful interactions with the external world. To obtain a subjective measure of the RHI, researchers typically ask participants to rate statements such as "I felt as if the rubber hand were my hand". Here we demonstrate how the crossmodal congruency task can be used to obtain an objective behavioral measure within this paradigm.
The variant of the crossmodal congruency task we employ involves the presentation of tactile targets and visual distractors. Targets and distractors are spatially congruent (i.e. same finger) on some trials and incongruent (i.e. different finger) on others. The difference in performance between incongruent and congruent trials - the crossmodal congruency effect (CCE) - indexes multisensory interactions. Importantly, the CCE is modulated both by viewing a hand as well as the synchrony of viewed and felt touch which are both crucial factors for the RHI.
The use of the crossmodal congruency task within the RHI paradigm has several advantages. It is a simple behavioral measure which can be repeated many times and which can be obtained during the illusion while participants view the artificial hand. Furthermore, this measure is not susceptible to observer and experimenter biases. The combination of the RHI paradigm with the crossmodal congruency task allows in particular for the investigation of multisensory processes which are critical for modulations of body representations as in the RHI.

We demonstrate how an objective measure can be employed in the widely employed rubber hand illusion paradigm. This measure is obtained by modifying the well-established crossmodal congruency task. This task allows the investigation of multisensory processes which are critical for modulations of body representations as in the rubber hand illusion.

The rubber hand illusion (RHI) is a popular experimental paradigm. Participants view touch on an artificial rubber hand while the participants' own hidden ...

Meal Duration as a Measure of Orofacial Nociceptive Responses in Rodents

A lengthening in meal duration can be used to measure an increase in orofacial mechanical hyperalgesia having similarities to the guarding behavior of humans with orofacial pain. To measure meal duration unrestrained rats are continuously kept in sound attenuated, computerized feeding modules for days to weeks to record feeding behavior. These sound-attenuated chambers are equipped with chow pellet dispensers. The dispenser has a pellet trough with a photobeam placed at the bottom of the trough and when a rodent removes a pellet from the feeder trough this beam is no longer blocked, signaling the computer to drop another pellet. The computer records the date and time when the pellets were taken from the trough and from this data the experimenter can calculate the meal parameters. When calculating meal parameters a meal was defined based on previous work and was set at 10 min (in other words when the animal does not eat for 10 min that would be the end of the animal&#39;s meal) also the minimum meal size was set at 3 pellets. The meal duration, meal number, food intake, meal size and inter-meal interval can then be calculated by the software for any time period that the operator desires. Of the feeding parameters that can be calculated meal duration has been shown to be a continuous noninvasive biological marker of orofacial nociception in male rats and mice and female rats. Meal duration measurements are quantitative, require no training or animal manipulation, require cortical participation, and do not compete with other experimentally induced behaviors. These factors distinguish this assay from other operant or reflex methods for recording orofacial nociception.

A lengthening in meal duration represents orofacial nociceptive behavior in rodents similar to the guarding behavior of humans with orofacial pain. Eating is a behavior that requires no training or animal manipulation, requires cortical participation, and is not competing with other experimentally induced behaviors, distinguishing this assay from alternative reflex or operant measurements.

A lengthening in meal duration can be used to measure an increase in orofacial mechanical hyperalgesia having similarities to the guarding behavior of ...

Assessment of Murine Exercise Endurance Without the Use of a Shock Grid: An Alternative to Forced Exercise

Using laboratory mouse models, the molecular pathways responsible for the metabolic benefits of endurance exercise are beginning to be defined. The most common method for assessing exercise endurance in mice utilizes forced running on a motorized treadmill equipped with a shock grid. Animals who quit running are pushed by the moving treadmill belt onto a grid that delivers an electric foot shock; to escape the negative stimulus, the mice return to running on the belt. However, avoidance behavior and psychological stress due to use of a shock apparatus can interfere with quantitation of running endurance, as well as confound measurements of postexercise serum hormone and cytokine levels. Here, we demonstrate and validate a refined method to measure running endurance in na&#239;ve C57BL/6 laboratory mice on a motorized treadmill without utilizing a shock grid. When mice are preacclimated to the treadmill, they run voluntarily with gait speeds specific to each mouse. Use of the shock grid is replaced by gentle encouragement by a human operator using a tongue depressor, coupled with sensitivity to the voluntary willingness to run on the part of the mouse. Clear endpoints for quantifying running time-to-exhaustion for each mouse are defined and reflected in behavioral signs of exhaustion such as splayed posture and labored breathing. This method is a humane refinement which also decreases the confounding effects of stress on experimental parameters.

A method to assess exercise endurance in laboratory mice without the use of a shock grid is demonstrated. This method is a humane refinement that can decrease the confounding effects of stress on experimental parameters.

Using laboratory mouse models, the molecular pathways responsible for the metabolic benefits of endurance exercise are beginning to be defined. The most ...

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of ...

A Protocol of Manual Tests to Measure Sensation and Pain in Humans

Numerous qualitative and quantitative techniques can be used to test sensory nerves and pain in both research and clinical settings. The current study demonstrates a quantitative sensory testing protocol using techniques to measure tactile sensation and pain threshold for pressure and heat using portable and easily accessed equipment. These techniques and equipment are ideal for new laboratories and clinics where cost is a concern or a limiting factor. We demonstrate measurement techniques for the following: cutaneous mechanical sensitivity on the arms and legs (von-Frey filaments), radiant and contact heat sensitivity (with both threshold and qualitative assessments using the Visual Analog Scale (VAS)), and mechanical pressure sensitivity (algometer, with both threshold and the VAS). The techniques and equipment described and demonstrated here can be easily purchased, stored, and transported by most clinics and research laboratories around the world. A limitation of this approach is a lack of automation or computer control. Thus, these processes can be more labor intensive in terms of personnel training and data recording than the more sophisticated equipment. We provide a set of reliability data for the demonstrated techniques. From our description, a new laboratory should be able to set up and run these tests and to develop their own internal reliability data.

The goal of this procedure is to demonstrate a battery of quantitative techniques for sensory and pain measurement in humans. The equipment and techniques described are commonly found in pain clinics or are easy to obtain.

Numerous qualitative and quantitative techniques can be used to test sensory nerves and pain in both research and clinical settings. The current study ...

Simultaneous Recordings of Cortical Local Field Potentials and Electrocorticograms in Response to Nociceptive Laser Stimuli from Freely Moving Rats

Electrocortical responses, elicited by laser heat pulses that selectively activate nociceptive free nerve endings, are widely used in many animal and human studies to investigate the cortical processing of nociceptive information. These laser-evoked brain potentials (LEPs) consist of several transient responses that are time-locked to the onset of laser stimuli. However, the functional properties of the LEP responses are still largely unknown, due to the lack of a sampling technique that can simultaneously record neural activities at the surface of the cortex (i.e., electrocorticogram [ECoG] and scalp electroencephalogram [scalp EEG]) and inside the brain (i.e., local field potential [LFP]). To address this issue, we present here an animal protocol using freely moving rats. This protocol is composed of three main procedures: (1) animal preparation and surgical procedures, (2) a simultaneous recording of ECoG and LFP in response to nociceptive laser stimuli, and (3) data analysis and feature extraction. Specifically, with the help of a 3D-printed protective shell, both ECoG and LFP electrodes implanted on the rat&#39;s skull were securely held together. During data collection, laser pulses were delivered on the rat&#39;s forepaws through gaps in the bottom of the chamber when the animal was in spontaneous stillness. Ongoing white noise was played to avoid the activation of the auditory system by the laser-generated ultrasounds. As a consequence, only nociceptive responses were selectively recorded. Using the standard analytical procedures (e.g., band-pass filtering, epoch extraction, and baseline correction) to extract stimulus-related brain responses, we obtained results showing that LEPs with a high signal-to-noise ratio were simultaneously recorded from ECoG and LFP electrodes. This methodology makes the simultaneous recording of ECoG and LFP activities possible, which provides a bridge of electrocortical signals at the mesoscopic and macroscopic levels, thereby facilitating the investigation of nociceptive information processing in the brain.

We developed a technique that simultaneously records both electrocorticography and local field potentials in response to nociceptive laser stimuli from freely moving rats. This technique helps establish a direct relationship of electrocortical signals at the mesoscopic and macroscopic levels, which facilitates the investigation of nociceptive information processing in the brain.

Electrocortical responses, elicited by laser heat pulses that selectively activate nociceptive free nerve endings, are widely used in many animal and ...

Multi-Modal Signals for Analyzing Pain Responses to Thermal and Electrical Stimuli

The assessment of pain relies mostly on methods that require a person to communicate. However, for people with cognitive and verbal impairments, existing methods are not sufficient as they lack reliability and validity. To approach this problem, recent research focuses on an objective pain assessment facilitated by parameters of responses derived from physiology, and video and audio signals. To develop reliable automated pain recognition systems, efforts have been made in creating multimodal databases in order to analyze pain and detect valid pain patterns. While the results are promising, they only focus on discriminating pain or pain intensities versus no pain. In order to advance this, research should also consider the quality and duration of pain as they provide additional valuable information for more advanced pain management. To complement existing databases and the analysis of pain regarding quality and length, this paper proposes a psychophysiological experiment to elicit, measure, and collect valid pain reactions. Participants are subjected to painful stimuli that differ in intensity (low, medium, and high), duration (5 s / 1 min), and modality (heat / electric pain) while audio, video (e.g., facial expressions, body gestures, facial skin temperature), and physiological signals (e.g., electrocardiogram [ECG], skin conductance level [SCL], facial electromyography [EMG], and EMG of M. trapezius) are being recorded. The study consists of a calibration phase to determine a subject&#8217;s individual pain range (from low to intolerable pain) and a stimulation phase in which pain stimuli, depending on the calibrated range, are applied. The obtained data may allow refining, improving, and evaluating automated recognition systems in terms of an objective pain assessment. For further development of such systems and to investigate pain reactions in more detail, additional pain modalities such as pressure, chemical, or cold pain should be included in future studies. Recorded data of this study will be released as the &#8220;X-ITE Pain Database&#8221;.

This article focuses on the experimental elicitation of pain through heat (thermal) and electrical stimulation while recording physiological, visual, and paralinguistic responses. It aims at collecting valid multimodal data for analyzing pain based on its intensity, quality, and duration.&#160;

The assessment of pain relies mostly on methods that require a person to communicate. However, for people with cognitive and verbal impairments, existing ...

Chronic Post-Ischemia Pain Model for Complex Regional Pain Syndrome Type-I in Rats

Complex regional pain syndrome type-I (CRPS-I) is a neurological disease that causes severe pain among patients and remains an unresolved medical condition. However, the underlying mechanisms of CRPS-I have yet to be revealed. It is known that ischemia/reperfusion is one of the leading factors that causes CRPS-I. By means of prolonged ischemia and reperfusion of the hind limb, the rat chronic post-ischemia pain (CPIP) model has been established to mimic CRPS-I. The CPIP model has become a well-recognized animal model for studying the mechanisms of CRPS-I. This protocol describes the detailed procedures involved in the establishment of the rat model of CPIP, including anesthesia, followed by ischemia/reperfusion of the hind limb. Characteristics of the rat CPIP model are further evaluated by measuring the mechanical and thermal hypersensitivities of the hind limb as well as the nocifensive responses to acute capsaicin injection. The rat CPIP model exhibits several CRPS-I-like manifestations, including hind limb edema and hyperemia in the early stage after establishment, persistent thermal and mechanical hypersensitivities, and increased nocifensive responses to acute capsaicin injection. These characteristics render it a suitable animal model for further investigation of the mechanisms involved in CRPS-I.

Provided here is a protocol that details steps to establish an animal model of chronic post-ischemia pain (CPIP). This is a well-recognized model mimicking human complex regional pain syndrome type-I. Mechanical and thermal hypersensitivities are further evaluated, as well as capsaicin-induced nocifensive behaviors observed in the CPIP rat model.

Complex regional pain syndrome type-I (CRPS-I) is a neurological disease that causes severe pain among patients and remains an unresolved medical ...

Operant Conditioning Task to Measure Song Preference in Zebra Finches

An operant conditioning paradigm is used to test the song preference of female zebra finches. Finches are placed in a two-chambered cage with a connecting opening and indicate their preference for a song by landing on a perch within each chamber. By interrupting the infrared beam from a photoelectric sensor above each perch, the bird activates the playback of a song through a speaker located on each side of the cage. Freely available software is used to trigger the song playback from each perch. To determine the song preference of each animal, her chamber preference is first identified by triggering no song playback when she lands on each perch. This chamber preference is then compared to her song preference. A minimum activity threshold is set to ensure the preference is real. Using this method, we show that paired females prefer the song of their partner. This method was used to understand the contribution of dopamine to the formation and maintenance of song preference.

We describe a technique to evaluate song preference in zebra finches. Females are placed in a two-chambered cage and song preference is measured by the number of times she triggers the playback of one song by landing on a perch within one chamber, compared with triggering a different song in the second chamber. Perch landings are counted using infrared sensors.

An operant conditioning paradigm is used to test the song preference of female zebra finches. Finches are placed in a two-chambered cage with a connecting ...

The Use of Traditional Fear Tests to Evaluate Different Emotional Circuits in Cattle

Animal temperament is complex and has implications for productivity and economic profitability. Quantifying an animal&#39;s response to differing stimuli may facilitate breeding selections and identify animals that are better suited to specific management strategies. Multiple tests have been developed to evaluate cattle temperament (e.g., exit velocity, chute score, pen score, open field test, startle test, bovine zero maze), but each of these tests evaluates the animal&#39;s response to different stimuli (e.g., isolation, novel environment, startle, willingness to enter an enclosed area). Cattle temperament has been observed to be relatively stable over time. However, the evaluation of temperament has the potential to be influenced by current conditions, previous experiences, and observer bias. Many of these temperament tests have been improperly categorized as fear tests and have also been criticized for being subjective. This paper provides a framework for standardizing behavioral tests for cattle and suggests that these different evaluations assess different aspects of the animal&#39;s overall temperament.

Here, we present a protocol to conduct a variety of behavioral tests in cattle that have been designed to evaluate emotions. A battery of behavioral tests (open field test, startle test, bovine zero maze, exit velocity, pen score, and chute score) were conducted to evaluate different components of animal temperament.

Animal temperament is complex and has implications for productivity and economic profitability. Quantifying an animal&#39;s response to differing stimuli ...