<a href="https://www.jove.com/video/4313">微妙な子宮内膜症の術中検出：腹膜整合性の喪失に伴う骨盤痛の検出および治療 ​​のための新たなパラダイム</a> ブルースA. Lessey 1、H·リー·ヒグドンⅢ1、サラE.ミラー2、トーマスA.価格3 産婦人科の1部、生殖内分泌不妊の部門、グリーン病院システム、病理学、デューク大学ヘルスシステム、産婦人科の3科、生殖内分泌不妊、デューク大学の部門の2部門腹膜の整合性の損失は、子宮内膜症の軽度のフォームを持つ女性の慢性骨盤痛を理解し、治療するための新たなパラダイムを提供し、容易に腹腔鏡検査の時に染料の術中点滴を用いて検出することができる。 ミツバチにおける触覚エアコンや触角サンプリング戦略の運動解析（ セイヨウミツバチ L.）</a> サミールMujagić、サイモンマイケルウルト、スベンHellbach発信、フォルカーデュール 生物サイバネティックス、CITEC - 認知的インタラクション技術 - センター·オブ·エクセレンス、ビーレフェルト大学このプロトコルでは、条件に触覚刺激にミツバチを活かしたとファインスケール触角サンプリングパターンの運動を解析するための2Dモーションキャプチャ技術を導入する方法を示しています。 <a href="https://www.jove.com/video/4320">アメフラシカリフォルニ生理的な動きと送りモータプログラムを引き出すと録音用体外準備中</a> ジェフリー·M·マクマナス1、ホイLU 1、ヒレル·J·チール1、2、3 生物学の1部門、ケースウエスタンリザーブ大学、神経科学、ケースウエスタンリザーブ大学、生体医工学、ケース·ウェスタン·リザーブ大学の3学科の2学科我々は、 アメフラシの送り装置で行動を供給さまざまな種類のを引き出すと観察しながら細胞外記録し、in vitroでの神経、筋肉、および個々の識別されたニューロンから刺激するテクニックを説明します。 <a href="https://www.jove.com/video/50081">ステップ成長PEG-ペプチドハイドロゲルからβ細胞のスフェロイドの発生と復旧</a> アサドRazaさん、建淨 生体医工学専攻、工学および技術のパーデュー学校、インディアナ大学 - パデュー大学インディアナポリスで次のプロトコルはステップで、膵β細胞をカプセル化するための技術を提供チオール - エン写真クリック反応により形成される成長PEG-ペプチドハイドロゲル。この材料プラットフォームは、細胞のカプセル化のためのcytocompatible微小環境を提供していますが、また、ヒドロゲル内に形成されたセル構造のユーザ制御の迅速な復旧を可能にするだけでなく。 <a href="https://www.jove.com/video/4400">蛍光 in situハイブリダイゼーションと組み合わせることで染色体の複製タイミング</a> レスリースミス、マシュー·セイヤー 生化学·分子生物学、騎士がん研究所、オレゴン健康科学大学の学科染色体の複製タイミングの解析のための定量的な方法が記載されている。この方法は、哺乳動物染色体の複製タイミングを評価するためのin situハイブリダイゼーション（FISH） の蛍光との組み合わせでBrdUの取り込みを利用しています。この手法は、番目の内に再配置および再配置非染色体の直接比較を可能にするE同じセル。 <a href="https://www.jove.com/video/4392">細菌性病因を調査するための強力な感染モデルとして昆虫ガレリアmellonella</a> ナリニRamarao、クリスティーナニールセン·ルルー、ディディエLereclus INRA、Micalis UMR1319、フランス大きいワックス蛾ハチノスツヅリガの幼虫の経口およびイントラhaemocolic感染が記載されている。この昆虫は昆虫だけでなく、哺乳類の日和見菌の病原因子を研究するために使用することができます。昆虫の飼育、感染および in vivo での解析例の方法が記載されている。

特別な利害関係は宣言されません。

december 2012: this month in jove

ここでは可視化実験（ゼウス）のジャーナルの2012年12月号からいくつかのハイライトです。 今月、スミスとセイヤーは、2つの古典的な方法-ブロモデオキシウリジン（BrdU）の取り込みとi nの蛍光in situハイブリダイゼーション（FISH）、個々の哺乳動物染色体の複製タイミングを調べるを兼ね備えています。 BrdUの取り込みの違いを分析することによって、私どもの著者がどのように染色体再配列を検出することができ、単一細胞の相同染色体の間で非同期レプリケーションを引き起こす。 Joveの神経科学、マクマナスら。カリフォルニアウミウシ（ アメフラシのcalifornica）の神経筋活動を研究し、授乳中にその供給装置の動き。私どもの著者が、スラグの口器を隔離給食プログラム（例えば、かむと口に入れて誤飲する等、誘導し、給餌時に神経、筋肉、および個々のニューロンの活動を分析する方法を私達に示しています。 t &quot;は&gt;またJoveの神経科学、Mujagić らインチ彼らはミツバチが自分のアンテナとは異なる金属表面をスキャンすることができますどこに、ハイブからラボにミツバチ（ セイヨウミツバチ ） を取るが、その後、研究者は、特定した後に蜂の砂糖水を供給触覚刺激。この時、触角タッチ特定の表面ミツバチは、砂糖水を見越して、そのproboscisesを拡張するために訓練している。私どもの著者が次に触角の動きの変化が連想または非結合触覚的学習にどのように対応するかを分析します。 Joveのバイオでは、RazaさんとLinは、架橋高分子ヒドロゲルにカプセル化されたβ細胞のスフェロイドを生成するための手順を示しています。凝集物を回収した後、私達の著者は、細胞と再生医療研究のための組織ベースの治療法を研究するために、β細胞のスフェロイドを使用することができます。 Joveの臨床およびトランスレーショナル医学では、Lessey らは、識別するために、メチレンブルー色素で使用されていますが子宮内膜症、骨盤痛、女性では他の泌尿生殖器の問題を引き起こす条件のように。メチレンブルーと腹膜の表面を染色することによって、私達の著者は、そうでなければ腹腔鏡検査中に見逃される可能性が微妙な子宮内膜症のある領域を特定することができます。これは私達の著者が大幅に子宮内膜​​症患者のために手術結果を向上させ、影響を受けるすべての組織を除去することができます。 Joveの免疫と感染症では、我々はまた、ハニカム蛾として知られているワックス蛾（ ハチノスツヅリガ ） は 、私たち哺乳動物と多くの共通点を持っていることを学ぶ。実際には、ワックス蛾は哺乳類の感染症に関連する微生物の病原因子を特徴づけるために使用することができ、複雑な自然免疫システムを持っています。 Ramarao らは幼虫を育てると病因アッセイ用の微生物や微生物の毒素でそれらを感染させるためにどのように私たちを示しています。 このプレビューはJoveの2012年12月号のちょうどいくつかの注目すべきビデオの記事をまとめたものです。フルレングスバージョン用これらおよびビデオの記事より多くのsは、下記をご覧ください<a href="http://www.jove.com">www.jove.comを</a> 。

Watch this Scientific Journal Video about December 2012: This Month in JoVE at JoVE.com

December 2012: This Month in JoVE

ここでは可視化実験（ゼウス）のジャーナルの2012年12月号からいくつかのハイライトです。

2012年12月：JOVEのこの月

Here are some highlights from the December 2012 issue of Journal of Visualized Experiments (JoVE).
This month, Smith and Thayer combine two classical ...

Research

JoVE Journal

Biology

8.4K ビュー.  Massachusetts Eye and Ear. ここでは可視化実験（ゼウス）のジャーナルの2012年12月号からいくつかのハイライトです。

ビデオ: December 2012: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

2012年8月：Joveのでは、今月

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

生物学

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012：In Reviewに年

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...