<a href="https://www.jove.com/video/4313">Hafif Endometriozis İntraoperatif Algılama: Periton Bütünlük Kaybı ile İlişkili Pelvik Ağrı Tespiti ve Tedavisi İçin Yeni Bir ​​Paradigma</a> Bruce A. Lessey 1, H. Lee Higdon III 1, Sara E. Miller 2, Thomas A. Fiyat 3 Kadın Hastalıkları ve Doğum Anabilim Dalı 1, Üreme Endokrinolojisi ve İnfertilite, Greenville Hastanesi Sistemi, 2. Bölümü Patoloji Anabilim Dalı, Duke Üniversitesi Sağlık Sistemi, Kadın Hastalıkları ve Doğum Anabilim Dalı 3, Üreme Endokrinolojisi ve İnfertilite Bölümü, Duke Üniversitesi Periton bütünlüğü kaybı anlamak ve kronik pelvik ağrı tedavi endometriozis hafif formları olan kadınlarda ve kolayca laparoskopi sırasında boya intraoperatif instilasyonu kullanılarak tespit edilebilir yeni bir paradigma sunmaktadır. Bal Arısı yılında Dokunsal Havalandırma Ve antennal Örnekleme Stratejileri Hareket Analizi (Apis mellifera L.)</a> Samir Mujagić, Simon Michael Würth, Sven Hellbach, Volker Dürr Biyolojik Sibernetik, CITEC - Bilişsel Etkileşim Teknoloji - Mükemmeliyet Merkezi, Bielefeld Üniversitesi Bu protokolü biz durumu dokunsal uyaranlara karşı bal arıları koşulmuş ve ince çaplı anten örnekleme deseni kinematik analiz için bir 2B hareket yakalama tekniği tanıtmak nasıl gösterir. <a href="https://www.jove.com/video/4320">Aplysia&#39;nın californica fizyolojik Hareketleri Besleme Motor Programlar ortaya çıkartılması ve kayıt için Vitro Hazırlık In An</a> Jeffrey M. McManus 1, Hui Lu 1, Hillel J. Chiel 1, 2, 3 Biyoloji Bölümü 1, Case Western Reserve Üniversitesi, Nörobilim Bölümü 2, Case Western Reserve Üniversitesi, Biyomedikal Mühendisliği 3. Bölümü, Case Western Reserve Üniversitesi Biz ekstraselüler kayıt ve Aplysia&#39;nın beslenmesi aygıtında davranışları beslenme farklı eliciting ve gözleyerek sinirler, kaslar, in vitro ve in bireysel tespit nöronlardan uyarmak için bir teknik tarif. <a href="https://www.jove.com/video/50081">Adım büyüme PEG-peptid Hidrojeller kaynaktan β-hücre sferoidlerin Üretimi ve Kurtarma</a> Asad Raza, Chien-Chi Lin Biyomedikal Mühendisliği Bölümü, Mühendislik ve Teknoloji Purdue Okulu, Indiana Üniversitesi - Indianapolis at Purdue University Aşağıdaki protokol adımda pankreatik β-hücreleri encapsulating için teknikler sağlarTiyol-ene fotoğrafı tıklama reaksiyonlar oluşturduğu büyüme PEG-peptid hidrojeller. Bu malzeme platformu hücre kapsülleme için cytocompatible mikroçevrede sunuyor, ama aynı zamanda hidrojellerin bünyesinde oluşturulan hücre yapılarının kullanıcı kontrollü hızlı iyileşme sağlar sadece. <a href="https://www.jove.com/video/4400">Floresan in situ hibridizasyon ile birlikte Kromozom çoğaltılıyor Zamanlama</a> Leslie Smith Mathew Thayer Biyokimya Anabilim Dalı ve Moleküler Biyoloji, Knight Kanser Enstitüsü, Oregon Sağlık ve Bilim Üniversitesi Kromozom replikasyonu zamanlama analizi için nicel bir yöntem tarif edilmektedir. Yöntemi memeli kromozom çoğaltma zamanlamasını değerlendirmek için in situ hibridizasyon (FISH) floresan birlikte BrdU dahil kullanır. Bu teknik th içinde düzenlenmeyecek ve un-yeniden düzenlenmiş kromozomun doğrudan karşılaştırma için izin verire Aynı hücrede. <a href="https://www.jove.com/video/4392">Bakteriyel Patogenez İnceleyecek Güçlü Enfeksiyon Model olarak Böcek Galleria mellonella</a> Nalini Ramarao, Christina Nielsen-Leroux, Didier Lereclus INRA, Micalis UMR1319, Fransa Büyük bal mumu güvesi Galleria mellonella larvalarının Ağız ve intra haemocolic enfeksiyon tanımlanmıştır. Bu böcek entomopatojenik hem de memeli fırsatçı bakteri virülans faktörü incelemek için kullanılabilir. Böcekler, enfeksiyon ve in vivo analiz yöntemleri içinde örnekleri yetiştirilmesi tarif edilmiştir.

Çıkar çatışması ilan etti.

december 2012: this month in jove

İşte görüntülenmiştir Deneyleri (Jove) Dergisi Aralık 2012 Sayı bazı olayları vardır. Bu ay, Smith ve Thayer iki klasik yöntem-bromodeoxyuridine (BrdU) Kuruluş ve i floresans n situ hibridizasyon (FISH)-bireysel memeli kromozom çoğaltma zamanlamasını incelemek birleştirir. BrdU kuruluş farklılıkları analiz ederek, bizim yazarlar kromozomal yeniden düzenlenmeleri, tek bir hücrenin Homolog kromozomlar arasındaki uyumsuz çoğaltma neden nasıl saptayabilir. Vallahi Nörobilim, McManus ve ark. Kaliforniya deniz salyangozu (Aplysia&#39;nın californica) nöromüsküler aktivite eğitim ve beslenme sırasında besleme cihazları hareketleri. Bizim yazarlar, sülük en ağız izole beslenme programları (örneğin, ısırma ve yutma gibi, neden ve beslenme sırasında sinirler, kaslar ve bireysel nöronların aktivitesini analiz nasıl bize göstermek. Onlar arılar kendi antenleri ile değişik metal yüzeylere tarayabileceği nereye&gt; Ayrıca Jove Nörobilim, Mujagić ark t &quot;., laboratuara kovandan bal arısı (Apis mellifera) alır. Ardından, araştırmacılar belirli sonra arılara şekerli su beslemek dokunsal uyaranlara. Bu onların antenleri dokunmatik özellikle yüzeyler. Bizim yazarlar anten hareketi değişiklikler birleşmeli veya ilişkisel olmayan dokunsal öğrenme karşılık nasıl analiz ettiğimizde arılar, şekerli su beklentisiyle, kendi proboscises uzatmak için eğitir. Vallahi Biyomühendislik yılında Raza ve Lin bir çapraz bağlı polimer hidrojel kapsüllü β-hücre sferoidler üretmek için bir yordam göstermek. Agrega atlattıktan sonra, bizim yazarlar hücre ve rejeneratif tıp, araştırma için doku-temelli tedaviler incelemek için β-hücre sferoidler kullanabilirsiniz. Vallahi Klinik &amp; Translational Medicine yılında Lessey ark. Olduğunu belirlemek metilen mavisi kullanabilirsinizendometriozis, pelvik ağrı ve kadınlarda diğer genitoüriner sorunlar neden olan bir durum olarak. Metilen mavisi ile peritoneal yüzeylerde boyama, bizim yazarlar aksi laparoskopi sırasında kaçırılmış olabilir ince endometriozis alanları belirleyebilir. Bu bizim yazarlar büyük ölçüde endometriozis hastaları için cerrahi sonuçları geliştirerek, etkilenen tüm doku kaldırmak için izin verir. Vallahi İmmünoloji ve Enfeksiyon, biz de petek güvesi gibi bilinen petek güvesi (Galleria mellonella), bize memeliler ile ortak bir yeri olduğunu öğreniyoruz. Aslında, petek güvesi memeli enfeksiyonları alakalı mikrobiyal virülans faktörleri tanımlamak için kullanılan karmaşık doğuştan gelen bağışıklık sistemleri vardır. Ramarao ark. Nasıl larvaları yükseltmek ve patogenezi deneyleri için mikrop veya mikrobiyal toksinler onları bulaştırmak için bize göstermek. Bu önizleme Jüpiter arasında Aralık 2012 sayısında sadece birkaç kayda değer video yazılar özetlemektedir. Tam uzunlukta sürümü içinBu ve video-haberler daha birçok s, ziyaret edin <a href="http://www.jove.com">www.jove.com</a> .

Watch this Scientific Journal Video about December 2012: This Month in JoVE at JoVE.com

December 2012: This Month in JoVE

İşte görüntülenmiştir Deneyleri (Jove) Dergisi Aralık 2012 Sayı bazı olayları vardır.

: Aralik 2012 Jove Bu Ay

Here are some highlights from the December 2012 issue of Journal of Visualized Experiments (JoVE).
This month, Smith and Thayer combine two classical ...

Research

JoVE Journal

Biology

8.4K Görüntüleme.  Massachusetts Eye and Ear. İşte görüntülenmiştir Deneyleri (Jove) Dergisi Aralık 2012 Sayı bazı olayları vardır.

Video: December 2012: This Month in JoVE

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Ağustos 2012: Vallahi Bu Ay

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Biyoloji

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

2012: İnceleme Bir Yıl

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...