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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

9.5K Views

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09:12 min

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September 10th, 2016

DOI :

10.3791/54696-v

September 10th, 2016

•

9,499 Views

Ansley Semack¹, Rabia U. Malik², Sivaraj Sivaramakrishnan¹

¹Genetics, Cell Biology, and Development, University of Minnesota, ²Department of Cell and Developmental Biology, University of Michigan

章

文字起こし

要約

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G Protein selective GPCR Conformations

FRET Sensors

Live Cell Fluorometer Assay

GPCR Interactions

G Protein Activation

Agonist Conditions

Receptor Confirmation

Cell Transfection

Protein Expression

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