Cell Culture Preparation and Transfection Conditions
2:41
Förster Resonance Energy Transfer (FRET) Sensor G Protein-selective G Protein-coupled Receptor (GPCR) Conformation Analysis
6:11
Results: Optimizing FRET Spectra Generation and Analysis
8:01
Conclusion
Transcription
The overall goal of this live cell Forster resonance energy transfer, or FRET based assay, is to assess G protein-selective G protein coupled receptor or GPCR conformations under different agonist conditions. This method can help answer key questi
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Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.