The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging
RNAi Interaction Screen to Modify a Canal lumen Phenotype for Identification of Genes Involved in Intracellular Lumen Extension
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Scoring Excretory Canal Phenotypes using Fluorescence Dissecting Microscopy
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Imaging the C. elegans Excretory Canal using Confocal Microscopy
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Results: GFP Labeling, RNAi and Microscopic Analysis of Excretory Canal Lumen Morphogenesis Phenotypes
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Conclusion
文字起こし
The overall goal of this combination of standard procedures is to demonstrate how the C.elegans excretory canal can be used for the in vivo analysis of unicellular tube, intracellular lumen, and de novo polarized membrane biogenesis. This method c
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The C. elegans excretory canal is a unique single-cell model for the visual in vivo analysis of de novo polarized membrane biogenesis. This protocol describes a combination of standard genetic/RNAi and imaging approaches, adaptable for the identification and characterization of molecules directing unicellular tubulogenesis, and apical membrane and lumen biogenesis.