JoVE Journal

Immunology and Infection

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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

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07:29 min

October 12th, 2017

October 12th, 2017

9,169 Views

0:05

Title

0:58

Day 1: Preparation of Controls and Laboratory-specific Quality Controls

2:10

Day 1: Separation of HDL Cholesterol using HDL Precipitation

3:07

Day 2: Fluorochrome Assay

5:17

Results: Cell-free Biochemical Fluorometric Assay Results

6:32

Conclusion

文字起こし

The overall goal of this experimental method is to determine the function of high density lipoprotein using a biochemical cell-free assay that measures the lipid peroxide content of a specific amount of HDL. This method can help answer key questio

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We describe here a fluorometric cell-free biochemical assay for determination of HDL-lipid peroxidation. This rapid and reproducible assay can be used to determine HDL function in large scale studies and can contribute to our understanding of HDL function in human disease.

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