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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

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04:41 min

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July 3rd, 2018

DOI :

10.3791/57950-v

July 3rd, 2018

•

7,988 Views

Louise Benning¹, Samuel Robinson¹^,², Marie Follo³, Lukas Andreas Heger¹, Daniela Stallmann¹, Daniel Duerschmied¹, Christoph Bode¹, Ingo Ahrens¹^,⁴, Marcus Hortmann¹

¹Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, ²Department of Medicine, Monash University, ³Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, ⁴Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

章

文字起こし

要約

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Digital PCR

Circulating MicroRNAs

Acute Myocardial Infarction

Cardiovascular Disease

Biomarkers

Quantitative Real time PCR

RNA Isolation

Reverse Transcription

Droplet Digital PCR

Master Mix Preparation

Sample Handling

Thermal Cycling

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