7.8K Views
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10:24 min
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August 24th, 2018
DOI :
August 24th, 2018
•0:04
Title
0:29
Culture Preparation
2:06
Expression and Purification of GFP-E90DOPA by a Biosynthetic System
4:25
Oligomerization of Purified GFP-E90DOPA and SDS-PAGE Analysis
5:47
Conjugation of GFP-E90DOPA with an Alkyne Probe by SPOCQ, Purification of the Labeled GFP
7:10
SDS-PAGE Analysis and Fluorescence Gel Scanning of Labeled GFP
8:21
Results: Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine
10:01
Conclusion
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