JoVE Journal

Immunology and Infection

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A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

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06:46 min

July 5th, 2022

July 5th, 2022

2,475 Views

0:04

Introduction

1:06

Create Linker‐Coupled Antigen Solution

2:36

Preparation of Antigen Labeled Serum and the 6‐Plex Plate

3:54

Serum/Antigen Incubates Transfer into the 6‐Plex Plate

4:42

Washing the 6‐Plex Plate and Reading Buffer Addition

5:11

Results: Comparison of Antibody Levels Study Between the Single and the 6‐Plex ECL Assay

5:59

Conclusion

文字起こし

In line with the urgent need for screening for type 1 diabetes, celiac disease, and coronavirus disease 2019, we developed a high throughput 6-Plex electrochemiluminescence assay to simultaneously detect all four islet autoantibodies, tissue transglutaminase autoantibodies, and antibodies to the receptor binding domain of severe acute respiratory syndrome coronavirus 2.

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