JoVE Journal

Immunology and Infection

This content is Free Access.

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

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11:52 min

January 27th, 2023

January 27th, 2023

2,124 Views

0:04

Introduction

0:56

Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Whole Blood

2:34

Enrichment of CD14+ Monocytes from PBMCs

4:20

Osteoclast Differentiation In Vitro

5:46

TRAP Staining for Osteoclasts

7:10

Actin Ring Fluorescent Staining

9:40

Results: Differentiation of Osteoclasts and Assessing the Function of Mature Osteoclasts

11:12

Conclusion

文字起こし

Osteoclasts are key bone-resorbing cells in the body. This protocol describes a reliable method for the in vitro differentiation of osteoclasts from human peripheral blood monocytes. This method can be used as an important tool to further understand osteoclast biology in homeostasis and in diseases.

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