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Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

2.2K Views

11:58 min

January 6th, 2023

January 6th, 2023

2,208 Views

0:05

Introduction

1:07

Isolation of Esophageal Progenitor Cells and Fibroblasts

5:02

Establishment and Culture of Esophageal Organoids

6:31

Organoid Processing for Whole Mount Staining

9:22

Results: Isolation of Progenitor Cells and Fibroblast Subpopulations, Organoid Co-Cultures, and Whole-Mount Staining of Fibroblast-Organoid Interactions

11:24

Conclusion

文字起こし

Organoid-fibroblast co-cultures provide a model to study the in vivo stem cell niche. Here, a protocol for esophageal organoid-fibroblast co-cultures is described. Additionally, whole mount imaging is used to visualize the fibroblast-organoid interaction.

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