efficient-isolation-protocol-for-b-t-lymphocytes-from-human-palatine

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Tonsils form a part of the immune system providing the first line of defense against inhaled pathogens. Usually the term &#8220;tonsils&#8221; refers to ...

Uppsala Biomedical Center, Uppsala University

In this research slaughtered sheep and goats were investigated during August 2003-July 2004 at a slaughterhouse in Masjed-Soleyman, South-Western Iran. The number of infested animals with Przhevalskiana larvae and their age and sex was recorded. Collected larvae from infested animals were stored in Alcohol-Glycerin solution and were studied to determine their morphological properties and species. The data was analyzed by the chi-square test. Rate of infestation was 0.2 and 5.3% in sheep and goats, respectively. Infestation rate in male and female goats and sheep was significantly different (p<0.05). Prevalence of hypodermosis in sheep was significantly different in spring and winter, while infestation prevalence in goats was significantly different in four seasons of the year. Myiasis was seen more frequent in 2-3 and 1-2-year-old age groups of sheep and goats, respectively. The causative agents of goat and sheep hypodermosis were Przhevalskiana aegagri and Przhevalskiana crossii. The onset of fly activity was from late June to mid July.

A survey of seasonal infestation with Przhevalskiana larvae in slaughtered goats and sheep in South-Western Iran.

The eukaryotic expression plasmid of M2e-Hsp70 is a candidate M2e-based DNA vaccine. In order to evaluate the immunization potential of this construct, Specific Pathogen Free chickens were immunized either intramuscularly or orally. Mutant Salmonella typhimurium was used as carrier for oral delivery of the M2e-Hsp70 construct. M2e-specific humoral and cellular responses were tested by ELISA and Lymphocyte Proliferation Assay, respectively. Our results indicate that both humoral and cellular immune responses are conferred against M2e-Hsp70 plasmid in either of the intramuscular or oral routes of administration; however, these responses are significantly higher in intramuscular injection in contrast to oral administration. When it comes to mass vaccination of commercial chicken flocks oral administration is preferred due to the ease of application as well as its capability of eliciting mucosal, humoral and cellular immune responses; so measurements should be taken to improve the immunization potency of our orally delivered DNA vaccine.

Farzaneh Assadian

Department of Medical Biochemistry and Microbiology

Uppsala Biomedical Center, Uppsala University

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Farzaneh Assadian

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Medical Biochemistry and Microbiology

Uppsala Biomedical Center, Uppsala University

Efficient Isolation Protocol for B and T Lymphocytes from Human Palatine Tonsils

Department of Medical Biochemistry and Microbiology