Stefan Junne
Chair of Bioprocess Engineering,
Department of Biotechnology,
Chair of Bioprocess Engineering, Department of Biotechnology
Technische Universität Berlin
Stefan Junne is currently group leader of the working groups “Smart Bioproduction Grids” and “Process Analytical Technologies” at the lab of bioprocess engineering, institute of biotechnology at TU Berlin (Germany). As chemical engineer by training, he started to work in the area of bioprocess engineering, in particular biofuels production, about 15 years ago. After Dr. Junne obtained his Ph.D. in 2010 in the area of systems biology and modeling, he meanwhile focuses on the development, experimental optimization and scale up of various microbial bioprocesses in the area of mono, co- and anaerobic mixed cultivation for the production of bulk and fine chemicals, food and feed components. Beside a development of tailored bioreactors, in particular rocking single-use reactors, the understanding of cell physiology and morphology and the interaction of cells to different environmental conditions is in the focus of research. Therefore, single-cell based methods for in line applications are applied to understand the impact of stress conditions, as they occur during scale up and which are mimicked in scale down bioreactors, on population heterogeneity and to control of co-cultures. Such concepts are applied within a wide range of microorganisms, like microalgae, yeast and bacteria as well as filamentous fungi. Finally, by combining bioreactor design and suitable process analytical technology, morphologic control is conducted and coupled to population balances, e.g. under varying shear force cultivation conditions and gradient formation like it appears in the liquid phase of large scale production.
Besides, another major aim of Dr. Junne's work within bioprocess optimization is also to broaden the range of applicable feedstock by suitable pre-treatment methods, analytical tools, reactor and process design in order to achieve regional closed material cycles. Economic and life cycles aspects are considered for the energetic and material use of biogenic raw and residual material to obtain a high degree of sustainability and applicability up to the large scale.
In situ removal and purification of biosurfactants by automated surface enrichment.
Applied microbiology and biotechnology Nov, 2008 | Pubmed ID: 18690427
Scale-down simulators for metabolic analysis of large-scale bioprocesses.
Current opinion in biotechnology Feb, 2010 | Pubmed ID: 20185293
High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor.
Microbial cell factories , 2010 | Pubmed ID: 20509968
Electrooptical monitoring of cell polarizability and cell size in aerobic Escherichia coli batch cultivations.
Journal of industrial microbiology & biotechnology Sep, 2010 | Pubmed ID: 20512396
Two-compartment method for determination of the oxygen transfer rate with electrochemical sensors based on sulfite oxidation.
Biotechnology journal Aug, 2011 | Pubmed ID: 21751399
A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: impact of oscillations on Bacillus subtilis fed-batch cultivations.
Biotechnology journal Aug, 2011 | Pubmed ID: 21751400
Glucose-limited high cell density cultivations from small to pilot plant scale using an enzyme-controlled glucose delivery system.
New biotechnology Jan, 2012 | Pubmed ID: 22100433
Cultivation of marine microorganisms in single-use systems.
Advances in biochemical engineering/biotechnology , 2014 | Pubmed ID: 23893044
Lactose autoinduction with enzymatic glucose release: characterization of the cultivation system in bioreactor.
Protein expression and purification Feb, 2014 | Pubmed ID: 24215862
Process inhomogeneity leads to rapid side product turnover in cultivation of Corynebacterium glutamicum.
Microbial cell factories Jan, 2014 | Pubmed ID: 24410842
Response of Corynebacterium glutamicum exposed to oscillating cultivation conditions in a two- and a novel three-compartment scale-down bioreactor.
Biotechnology and bioengineering Jun, 2015 | Pubmed ID: 25728062
An improved HPLC-DAD method for clavulanic acid quantification in fermentation broths of Streptomyces clavuligerus.
Journal of pharmaceutical and biomedical analysis Feb, 2016 | Pubmed ID: 26760242
Inversion of the stereochemical configuration (3S, 5S)-clavaminic acid into (3R, 5R)-clavulanic acid: A computationally-assisted approach based on experimental evidence.
Journal of theoretical biology Apr, 2016 | Pubmed ID: 26835563
Life cycle assessment of flexibly fed biogas processes for an improved demand-oriented biogas supply.
Bioresource technology Nov, 2016 | Pubmed ID: 27522120
Tools for the determination of population heterogeneity caused by inhomogeneous cultivation conditions.
Journal of biotechnology Jun, 2017 | Pubmed ID: 28400137
Micro-Electromechanical Affinity Sensor for the Monitoring of Glucose in Bioprocess Media.
International journal of molecular sciences Jun, 2017 | Pubmed ID: 28594350
Sterol synthesis and cell size distribution under oscillatory growth conditions in Saccharomyces cerevisiae scale-down cultivations.
Yeast (Chichester, England) 02, 2018 | Pubmed ID: 28940694
Streptomyces clavuligerus shows a strong association between TCA cycle intermediate accumulation and clavulanic acid biosynthesis.
Applied microbiology and biotechnology May, 2018 | Pubmed ID: 29523936
How scalable and suitable are single-use bioreactors?
Current opinion in biotechnology 10, 2018 | Pubmed ID: 29753977
Real-time monitoring of the budding index in Saccharomyces cerevisiae batch cultivations with in situ microscopy.
Microbial cell factories May, 2018 | Pubmed ID: 29764434
Importance of the cultivation history for the response of Escherichia coli to oscillations in scale-down experiments.
Bioprocess and biosystems engineering Sep, 2018 | Pubmed ID: 29808419
Comparison of time-gated surface-enhanced raman spectroscopy (TG-SERS) and classical SERS based monitoring of Escherichia coli cultivation samples.
Biotechnology progress 11, 2018 | Pubmed ID: 29882305
Rocking Aspergillus: morphology-controlled cultivation of Aspergillus niger in a wave-mixed bioreactor for the production of secondary metabolites.
Microbial cell factories Aug, 2018 | Pubmed ID: 30129427
CFD predicted pH gradients in lactic acid bacteria cultivations.
Biotechnology and bioengineering Apr, 2019 | Pubmed ID: 30450609
Electrooptical Determination of Polarizability for On-Line Viability and Vitality Quantification of Cultures.
Frontiers in bioengineering and biotechnology , 2018 | Pubmed ID: 30564571
Degradation Kinetics of Clavulanic Acid in Fermentation Broths at Low Temperatures.
Antibiotics (Basel, Switzerland) Jan, 2019 | Pubmed ID: 30658482
Carboxylic acid consumption and production by Corynebacterium glutamicum.
Biotechnology progress May, 2019 | Pubmed ID: 30851150
Data of clavulanic acid and clavulanate-imidazole stability at low temperatures.
Data in brief Apr, 2019 | Pubmed ID: 31372423
Characterization of the Metabolic Response of to Shear Stress in Stirred Tanks and Single-Use 2D Rocking Motion Bioreactors for Clavulanic Acid Production.
Antibiotics (Basel, Switzerland) Sep, 2019 | Pubmed ID: 31569725
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