accumulation-distribution-fluorescent-microplastics-early-life-stages

Accumulation and Distribution of Fluorescent Microplastics in the Early Life Stages of Zebrafish

As a new type of environmental pollutant, microplastic has been widely found in the aquatic environment and poses a high threat to aquatic organisms. The ...

Zhejiang University of Technology

The ability to restore tissue function and morphology after injury is a key advantage of many fish for a greater chance of survival. The tissue regeneration process is regulated by multiple pathways, and it can therefore be hypothesized that environmental contaminants targeting components of these signaling pathways, may disrupt the fish's capability to repair or regenerate. This could lead to higher mortality and eventually even to a decline in populations. In this study, the effects of 17α‑ethinylestradiol (EE2), a synthetic estrogen, were assessed on the regenerative capacity of larval zebrafish. Zebrafish aged 2 hour post fertilization (hpf) were exposed to 1, 10, or 100 ng/L EE2, and the caudal fins were amputated at 72 hpf. It was found that EE2 exposure significantly inhibited fin regeneration and changed locomotor behavior. The transcription levels for most of the genes involved in the signaling networks regulating the fin regeneration, such as axin2, fgfr1, bmp2b and igf2b, were down-regulated in the amputated fish in response to EE2 exposure, which was in contrast to their increased patterns in the vehicle-exposed control fish. Additionally, the mRNA levels of several immune-related genes, such as il-1β, il-6, il-10 and nf-κb2, were significantly decreased after EE2 exposure, accompanied by a lower density of neutrophils migrated into the wound site. In conclusion, the present study indicated for the first time that estrogenic endocrine disrupting chemicals (EEDCs) could inhibit the regenerative capacity of zebrafish, and this effect was speculated to be mediated through the alteration in regeneration-related signaling pathways and immune competence. This work expands our knowledge of the potential effects of EEDCs on injured aquatic organisms, and highlights the ecotoxicological significance of relationships between regenerative process and endocrine system. This study also implies the potential application of fin regeneration assay for assessing immunotoxicity in ecotoxicological risk assessment.

Effects of 17α‑ethinylestradiol on caudal fin regeneration in zebrafish larvae.

Microplastic pollution is pervasive in aquatic environments, but the potential effects of microplastics on aquatic organisms are still under debate. Given that tissue damage is unavoidable in fish and the available data mostly concentrate on healthy fish, there is a large chance that the ecotoxicological risk of microplastic pollution is underrated. Therefore, in this study, the effects of microplastics on the regenerative capacity of injured fish were investigated using a zebrafish caudal fin regeneration model. After fin amputation at 72 h post fertilization, the larvae were exposed to polystyrene microplastics (0.1-10 mg/L) with diameters of 50 or 500 nm. Microplastic exposure significantly inhibited fin regeneration, both morphologically and functionally. Furthermore, the signaling networks that regulate fin regeneration, as well as reactive oxygen species signaling and the immune response, both of which are essential for tissue repair and regeneration, were altered. Transcriptomic analyses of the regenerating fin confirmed that genes related to fin regeneration were transcriptionally modulated in response to microplastic exposure and that metabolic pathways were also extensively involved. In conclusion, this study demonstrated for the first time that microplastic exposure could disrupt the regenerative capacity of fish and might eventually impair their fitness in the wild.

Inhibitory effects of polystyrene microplastics on caudal fin regeneration in zebrafish larvae.

Environmental pollutants are ubiquitous in global aquatic ecosystems and may cause immunotoxicity in aquatic organisms. However, disadvantages remain in the existing in vivo immunotoxicological methods, which make it difficult to meet the increasing demands for screening and for discriminating the immunotoxicity of environmental pollutants. In this study, the immune response in zebrafish eleutheroembryo was activated by acupuncture of the caudal fin at 72 hours post fertilization (hpf), and this immune model was further validated with a well-defined immunosuppressor, beclomethasone dipropionate (BDP). It was shown that acupuncture resulted in no increase in mortality in zebrafish eleutheroembryos. The transcription and protein levels of most immune genes were significantly increased after acupuncture, which indicated that acupuncture can effectively activate the immune response in zebrafish eleutheroembryos. Following exposure to BDP (0.01-1 μmol/L), the suppressive effects on the immune system were more significant in zebrafish that received acupuncture than in zebrafish that did not receive acupuncture. Considering these advantages, including its sensitivity, safety, and simple operation, over existing methods, the established immune model of zebrafish is promising for assessing the immunotoxicity of environmental pollutants.

Linqi Gu

College of Environment

Zhejiang University of Technology

Accumulation and Distribution of Fluorescent Microplastics in the Early Life Stages of Zebrafish