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National Taiwan University Hospital

5 ARTICLES PUBLISHED IN JoVE

Developmental Biology

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

Wei-Ting Ho^1,2, Chien-Chia Su³, Jung-Shen Chang³, Shu-Wen Chang¹, Fung-Rong Hu³, Tzuu-Shuh Jou^2,4, I-Jong Wang^3,4

¹Department of Ophthalmology, Far Eastern Memorial Hospital, ²Institute of Clinical Medicine, National Taiwan University, ³Department of Ophthalmology, National Taiwan University Hospital, ⁴College of Medicine, National Taiwan University

A primary culture of bovine corneal endothelial cells was used to investigate the mechanism of corneal endothelial-mesenchymal transition. Furthermore, a rat corneal endothelium cryoinjury model was used to demonstrate corneal endothelial-mesenchymal transition in vivo.

Education

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Kang-Yi Su^*1,2, Steven D. Goodman^*3, Hung-Ming Lai¹, Rong-Syuan Yen¹, Wei-Yao Hu¹, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Woei-Horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University

A non-labeled, non-radio-isotopic method for DNA polymerase proofreading and a DNA repair assay was developed by using high-resolution MALDI-TOF mass spectrometry and a single nucleotide extension strategy. The assay proved to be very specific, simple, rapid, and easy to perform for proofreading and repair patches shorter than 9-nucleotides.

Behavior

Computerized Adaptive Testing System of Functional Assessment of Stroke

Gong-Hong Lin¹, Yi-Jing Huang¹, Yeh-Tai Chou², Hsin-Yu Chiang³, Ching-Lin Hsieh^1,4,5

¹School of Occupational Therapy, College of Medicine, National Taiwan University, ²Research Center for Psychological and Educational Testing, National Taiwan Normal University, ³Department of Occupational Therapy, College of Medicine, Fu Jen Catholic University, ⁴Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital, ⁵Department of Occupational Therapy, College of Medical and Health Science, Asia University

Here, we present a protocol to develop the computerized adaptive testing system of the functional assessment of stroke (CAT-FAS). The CAT-FAS can simultaneously assess four functions (two motor functions [upper and lower extremities], postural control, and basic activities of daily living) with sufficient reliability and administrative efficiency.

Biochemistry

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Hui-Lan Chang^*1, Kang-Yi Su^*1,2, Steven D. Goodman³, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Sui-Yuan Chang^1,2, Woei-horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University

A non-labeled, non-radio-isotopic method to assay uracil-DNA glycosylase activity was developed using MALDI-TOF mass spectrometry for direct apurinic/apyrimidinic site-containing product analysis. The assay proved to be quite simple, specific, rapid, and easy to use for DNA glycosylase measurement.

Immunology and Infection

A Clinically Relevant Murine Model of Peritoneal Fibrosis by Dialysate and Catheters

Yi-Ting Chen^1,2,3, Shuei-Liong Lin^1,2,3

¹Department of Integrated Diagnostics & Therapeutics, National Taiwan University Hospital, ²Renal Division, Department of Internal Medicine, National Taiwan University Hospital, ³Graduate Institute of Physiology, National Taiwan University College of Medicine

Patients with severe peritoneal fibrosis have high morbidity and mortality. The mechanism of peritoneal fibrosis is unclear. In this study, we describe a simple experimental murine model of peritoneal fibrosis induced by the injection of peritoneal dialysis fluid.