Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napusVeronika Jedličková 1, Marie Štefková 1, Marek Sedláček 1, Klára Panzarová 2, Helene S. Robert 1
1Hormonal Crosstalk in Plant Development, Mendel Center for Plant Genomics and Proteomics, CEITEC MU-Central European Institute of Technology, Masaryk University, 2PSI - Photon Systems Instruments
The protocol describes hairy root induction using Arabidopsis primary inflorescence stems and Brassica napus hypocotyls. The hairy roots can be cultured and used as explants to regenerate transgenic plants.
