Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension CellsKimberly L. Pietersz 1, Paul J.H. Nijhuis 1, Matthijs H.M. Klunder 1, Joëlle van den Herik 1, Barbara Hobo 1, Fred de Winter 1, Joost Verhaagen 1,2
1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam
Here, a suspension HEK293 cell-based AAV production protocol is presented, resulting in reduced time and labor needed for vector production using components that are available for research purposes from commercial vendors.
