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Royal Netherlands Academy of Arts and Sciences (KNAW)

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells

Kimberly L. Pietersz¹, Paul J.H. Nijhuis¹, Matthijs H.M. Klunder¹, Joëlle van den Herik¹, Barbara Hobo¹, Fred de Winter¹, Joost Verhaagen^1,2

¹Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences (KNAW), ²Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

Here, a suspension HEK293 cell-based AAV production protocol is presented, resulting in reduced time and labor needed for vector production using components that are available for research purposes from commercial vendors.