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Temple University, Lewis Katz School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

Elizabeth Abraham¹, Mikel Zubillaga¹, Thomas Roule², Eleonora Stronati³, Naiara Akizu², Conchi Estaras¹

¹Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center, Temple University, Lewis Katz School of Medicine, ²Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, ³Department of Child and Adolescence Psychiatry, Children's Hospital of Philadelphia

This paper establishes a pipeline for high-quality single-cell and nuclei suspensions of gastrulating mouse embryos for sequencing of single cells and nuclei.