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Lewis & Clark College

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Super-resolution Imaging of Neuronal Dense-core Vesicles

Bethe A. Scalettar^1,2, Daniel Shaver², Stefanie Kaech³, Janis E. Lochner^2,4

¹Department of Physics, Lewis & Clark College, ²Program in Biochemistry and Molecular Biology, Lewis & Clark College, ³Jungers Center for Neuroscience Research, Oregon Health & Science University, ⁴Department of Chemistry, Lewis & Clark College

We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

Developmental Biology

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

Zoe T. Cook¹, Nicole L. Brockway¹, Tamily A. Weissman¹

¹Biology Department, Lewis & Clark College

In vivo imaging is a powerful tool that can be used to investigate the cellular mechanisms underlying nervous system development. Here we describe a technique for using time-lapse confocal microscopy to visualize large numbers of multicolor Brainbow-labeled cells in real time within the developing zebrafish nervous system.