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Shenzhen university

10 ARTICLES PUBLISHED IN JoVE

Engineering

Atmospheric Pressure Fabrication of Large-Sized Single-Layer Rectangular SnSe Flakes

Jizhou Jiang^1,2, Calvin Pei Yu Wong^2,3, Wenjing Zhang¹, Andrew Thye Shen Wee^2,4

¹SZU-NUS Collaborative Innovation Center for Optoelectronic Science & Technology, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, ²Department of Physics, National University of Singapore, ³NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences, ⁴Centre for Advanced 2D Materials and Graphene Research Centre, National University of Singapore

A protocol is presented demonstrating a two-step fabrication technique to grow large-sized single-layer rectangular shaped SnSe flakes on low-cost SiO₂/Si dielectrics wafers in an atmospheric pressure quartz tube furnace system.

Biology

A Method for Islet Transplantation to the Omentum in Mouse

Ying Lu^*1, Shangyou Zou^*1, Suzanne Bertera^*2, Rita Bottino², David K.C. Cooper³, Zhengzhao Liu¹, Yi Huang¹, Chengjun Wang¹, Chungu Hong¹, Tian He¹, Hancheng Zhang¹, Qin Huo⁴, Hongxing Fu⁵, Zhiming Cai¹, Lisha Mou¹

¹Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen University Health Science Center, ²Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, ³Xenotransplantation Program/Department of Surgery, The University of Alabama at Birmingham, ⁴College of Life Science and Oceanography, Shenzhen University, ⁵School of Pharmcy, Wenzhou Medical College

A method for the omental transplantation of islets in a mouse is introduced. The isolated islets are mixed with hydrogel and the mixture is placed into the omental pouch of a diabetic mouse. Then, the blood glucose is monitored, and immuno-histochemical analysis is performed.

Behavior

The Modified Temptation Resistance Task: A Paradigm to Elicit Children's Strategic Lie-telling

Lamei Wang¹, Zhenlin Wang²

¹College of Psychology and Sociology, Shenzhen University, ²Department of Psychology, The Education University of Hong Kong

The protocol for the temptation resistance paradigm was designed to elicit 2- to 8-year-old children's strategic lie-telling behaviors. The reward of transgression was intended to be too tempting to resist, so that children's spontaneous lie-telling behavior in the presence of irreversible evidence due to the transgression could be observed.

JoVE Core

Applicability Analysis of Assessment Methods for Morphological Parameters of Corroded Steel Bars

Dawang Li^1,2, Ping Li^1,2, Yingang Du³, Ren Wei^1,2

¹Department of Civil Engineering, Shenzhen University, ²Guangdong Province Key Laboratory of Durability for Marine Civil Engineering, Shenzhen University, ³Department of Engineering and the Built Environment, Anglia Ruskin University

This paper measures the geometry and the amount of corrosion of a steel bar using different methods: mass loss, calipers, drainage measurements, 3D scanning, and X-ray micro-computed tomography (XCT).

Neuroscience

How to Calculate and Validate Inter-brain Synchronization in a fNIRS Hyperscanning Study

Yinying Hu¹, Zixuan Wang¹, Bei Song², Yafeng Pan³, Xiaojun Cheng⁴, Yi Zhu¹, Yi Hu¹

¹Institute of Brain and Education Innovation, School of Psychology and Cognitive Science, East China Normal University, ²Department of Musicology, Harbin Conservatory of Music, ³Department of Clinical Neuroscience, Karolinska Institutet, ⁴School of Psychology, Shenzhen University

The dynamics between coupled brains of individuals have been increasingly represented by inter-brain synchronization (IBS) when they coordinate with each other, mostly using simultaneous-recording signals of brains (namely hyperscanning) with fNIRS. In fNIRS hyperscanning studies, IBS has been commonly assessed through the wavelet transform coherence (WTC) method because of its advantage on expanding time series into time-frequency space where oscillations can be seen in a highly intuitive way. The observed IBS can be further validated via the permutation-based random pairing of the trial, partner, and condition. Here, a protocol is presented to describe how to obtain brain signals via fNIRS technology, calculate IBS through the WTC method, and validate IBS by permutation in a hyperscanning study. Further, we discuss the critical issues when using the above methods, including the choice of fNIRS signals, methods of data preprocessing, and optional parameters of computations. In summary, using the WTC method and permutation is a potentially standard pipeline for analyzing IBS in fNIRS hyperscanning studies, contributing to both the reproducibility and reliability of IBS.

JoVE Core

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Yiwei Zhao¹, Gene Chi Wai Man^1,2, Loucia Kit Ying Chan¹, Xi Guo¹, Yingyu Liu³, Tao Zhang¹, Joseph Kwong^1,4, Chi Chiu Wang^1,5, Xiaoyan Chen³, Tin Chiu Li¹

¹Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, ²Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, ³Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, ⁴School of Medicine, Faculty of Medicine and Health Sciences, Keele University, ⁵Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

Despite the advancements in multiplex immunohistochemistry and multispectral imaging, characterizing the density and clustering of major immune cells simultaneously in the endometrium remains a challenge. This paper describes a detailed multiplex staining protocol and imaging for the simultaneous localization of four immune cell types in the endometrium.

Neuroscience

Modulation of the Neurophysiological Response to Fearful and Stressful Stimuli Through Repetitive Religious Chanting

Hin Hung Sik¹, Georgios T. Halkias¹, Chunqi Chang², Junling Gao¹, Hang Kin Leung¹, Bonnie Wai Yan Wu¹

¹Buddhism and Science Research Lab, Centre of Buddhist Studies, The University of Hong Kong, ²School of Biomedical Engineering, Shenzhen University

The present event-related potential (ERP) study provides a unique protocol for investigating how religious chanting can modulate negative emotions. The results demonstrate that the late positive potential (LPP) is a robust neurophysiological response to negative emotional stimuli and can be effectively modulated by repetitive religious chanting.

Immunology and Infection

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Liang Wang^*1,2,3, Jin-Xin Lai^*1, Yu-Ting Si^*1,4, Xu-Xia Cui^1,4, Zeeshan Umar^1,5, Xiao-Jun Ru¹, Xin-Yu Zhang¹, Zheng-Kang Li¹, Alfred Chin Yen Tay^5,6,7,8, Barry J. Marshall^5,6,7,8, Guang-Hua Li¹, Bing Gu¹

¹Laboratory Medicine, Guangdong Provincial People’s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, ²Division of Microbiology and Immunology, School of Biomedical Sciences, The University of Western Australia, ³Center for Precision Health, School of Medical and Health Sciences, Edith Cowan University, ⁴Medical Technology School of Xuzhou Medical University, ⁵Marshall Laboratory of Biomedical Engineering, School of Medicine, Shenzhen University, ⁶The Marshall Centre for Infectious Diseases Research and Training, The University of Western Australia, ⁷Marshall International Digestive Diseases Hospital, Zhengzhou University, ⁸Marshall Medical Research Center, Fifth Affiliated Hospital of Zhengzhou University

The protocol presents a noninvasive method for the rapid diagnosis of Helicobacter pylori stomach infections through the string test and determines its antibiotic resistance to clarithromycin and levofloxacin using quantitative polymerase chain reaction (qPCR).

Immunology and Infection

Accessing Early Differentiation of Virus-Specific Follicular Helper CD4⁺ T Cell in Acute LCMV-Infected Mice

Yao Lin^*1,2, Shuai Yue^*3, Yang Yang^*4, Junjian He⁵, Xiaofan Yang⁶, Lilin Ye⁵, Xiangyu Chen⁷

¹Department of Urology, South China Hospital, Medical School, Shenzhen University, ²Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, ³Cancer Center, Daping Hospital & Army Medical Center of PLA, Third Military Medical University, ⁴Guangdong Provincial Key Laboratory of Immune Regulation and Immunotherapy, School of Laboratory Medicine and Biotechnology, Southern Medical University, ⁵Institute of Immunology, Third Military Medical University, ⁶Dermatology Hospital, Southern Medical University, ⁷Institute of Immunological Innovation and Translation, Chongqing Medical University

The current study showcases protocols for assessing the early fate commitment of virus-specific T_FH cells and manipulating gene expression in these cells.

Biology

Co-culture Model Using Two Types of Adherent Cell Lines

Zhuo Song^*1, Lisha Zhao^*1, Jingwen Hu^*1, Xi Zheng², Yingyu Liu¹, Hao Wang¹, Xiaoyan Chen¹

¹Maternal-Fetal Medicine Institute, Department of Obstetrics and Gynecology, Shenzhen Baoan Women's and Children's Hospital, Shenzhen University, ²The First Clinical Medicine College, Southern Medical University

The protocol shows a novel in vitro experimental model that can recapitulate the biology of two kinds of adherent cell lines with a three-dimensional (3D)-printed scaffold. The construction of this model and operating procedures, from cell preparation and cell culture to analysis and evaluation, are described.