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University Of Torino

3 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Oxidative Stress in Zebrafish Embryos

Vera Mugoni¹, Annalisa Camporeale¹, Massimo M. Santoro^1,2

¹Department of Molecular Biotechnology and Health Science, University of Torino, ²Laboratory of Endothelial Molecular Biology, Vesalius Research Center, VIB

Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.

Bioengineering

Transport Properties of Ibuprofen Encapsulated in Cyclodextrin Nanosponge Hydrogels: A Proton HR-MAS NMR Spectroscopy Study

Monica Ferro^*1, Franca Castiglione^*1, Carlo Punta¹, Lucio Melone^1,2, Walter Panzeri³, Barbara Rossi⁴, Francesco Trotta⁵, Andrea Mele¹

¹Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, ²Università degli studi e-Campus, ³ICRM, CNR - Consiglio Nazionale delle Ricerche, ⁴Elettra Sincrotrone Trieste, ⁵Department of Chemistry, University of Torino

The motion regimes of ibuprofen encapsulated in β-cyclodextrin nanosponges polymer network are investigated using pulsed-field-gradient spin-echo (PGSE) NMR technique. Synthesis, purification, drug loading, implementation of the NMR pulse sequence and data analysis to work out the mean square displacement of the drug at several observation times are described in detail.

Modified Lemaire Lateral Extraarticular Tenodesis Using an Anterior Cruciate Ligament Reconstruction Suspensory Device

Marco Bechis¹, Roberto Rossi¹, Davide E. Bonasia¹

¹AO Ordine Mauriziano Hospital, Department of Orthopedics and Traumatology, University of Torino

This article describes a modified Lemaire technique that helps achieve a stable and functional anterolateral plasty during ACL reconstruction without additional femoral tunnels or fixation devices.