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Centre For Addiction And Mental Health

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Julie Winterburn^1,2, Jens C. Pruessner³, Chavez Sofia^4,5, Mark M. Schira^6,7, Nancy J. Lobaugh^4,8, Aristotle N. Voineskos^5,9, M. Mallar Chakravarty^1,2

¹Institute of Biomaterials and Biomedical Engineering, University of Toronto, ²Computational Brain Anatomy Laboratory, Douglas Institute, McGill University, ³McGill Centre for Studies in Aging, McGill University, ⁴MRI Unit, Research Imaging Centre, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, ⁵Department of Psychiatry, University of Toronto, ⁶School of Psychology, University of Wollongong, ⁷Neuroscience Research Australia, ⁸Department of Medicine, University of Toronto, ⁹Kimel Family Translational Imaging Genetics Research Laboratory, Research Imaging Centre, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health

The goal of this manuscript is to study the hippocampus and hippocampal subfields using MRI. The manuscript describes a protocol for segmenting the hippocampus and five hippocampal substructures: cornu ammonis (CA) 1, CA2/CA3, CA4/dentate gyrus, strata radiatum/lacunosum/moleculare, and subiculum.

Medicine

MRI-guided dmPFC-rTMS as a Treatment for Treatment-resistant Major Depressive Disorder

Katharine Dunlop¹, Pauline Gaprielian⁶, Daniel Blumberger^5,7, Zafiris J. Daskalakis^5,7, Sidney H. Kennedy^2,3,5, Peter Giacobbe^2,3,5, Jonathan Downar^2,3,4,5

¹Institute of Medical Sciences, University of Toronto, ²MRI-Guided rTMS Clinic, University Health Network, ³Department of Psychiatry, University Health Network, ⁴Toronto Western Research Institute, University Health Network, ⁵Department of Psychiatry, University of Toronto, ⁶Faculty of Arts and Science, University of Toronto, ⁷Temerty Centre for Therapeutic Brain Intervention, Centre for Addiction and Mental Health

Here we outline the procedure for MRI-guided repetitive transcranial magnetic stimulation to the dorsomedial prefrontal cortex as an experimental treatment for major depressive disorder.

JoVE Core

Combined Transcranial Magnetic Stimulation and Electroencephalography of the Dorsolateral Prefrontal Cortex

Pantelis Lioumis¹, Reza Zomorrodi¹, Itay Hadas¹, Zafiris J. Daskalakis^1,2, Daniel M. Blumberger^1,2

¹Temerty Centre for Therapeutic Brain Intervention at the Centre for Addiction and Mental Health, ²Department of Psychiatry and Institute of Medical Science, Faculty of Medicine, University of Toronto

The protocol presented here is for TMS-EEG studies utilizing intracortical excitability test-retest design paradigms. The intent of the protocol is to produce reliable and reproducible cortical excitability measures for assessing neurophysiological functioning related to therapeutic interventions in the treatment of neuropsychiatric diseases such as major depression.

Neuroscience

A Visual Approach for Inducing Dolichoectasia in Mice to Model Large Vessel-Mediated Cerebrovascular Dysfunction

Dominic Simpson^1,2, Christopher D. Morrone¹, Darcy Wear^1,2, Jose Gutierrez³, Wai Haung Yu²

¹Brain Health Imaging Centre, Centre for Addiction and Mental Health, ²Department of Pharmacology and Toxicology, University of Toronto, ³Department of Neurology, Columbia University

We demonstrate chemically inducing large blood vessel dilatation in mice as a model for investigating cerebrovascular dysfunction, which can be used for vascular dementia and Alzheimer's disease modeling. We also demonstrate visualizing the vasculature by injecting silicone rubber compound and providing clear visual guidance for measuring changes in blood vessel size.