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University of Toronto Mississauga

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Quantification of Visual Feature Selectivity of the Optokinetic Reflex in Mice

¹Department of Biology, University of Toronto Mississauga, ²Department of Cell and Systems Biology, University of Toronto

Here, we describe a standard protocol for quantifying the optokinetic reflex. It combines virtual drum stimulation and video-oculography, and thus allows precise evaluation of the feature selectivity of the behavior and its adaptive plasticity.

Bioengineering

Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Alexander Duggan¹, David McMillen¹

¹University of Toronto Mississauga

Here, we present protocols for working with Limosilactobacillus reuteri DSM20016, detailing growth, plasmid transformation, colony PCR, fluorescent reporter protein measurement, and limited plasmid mini-prep, as well as common issues and troubleshooting. These protocols allow the measurement of reporter proteins in DSM20016, or confirmation via colony PCR if no reporter is involved.