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Deakin University

4 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Measuring Oral Fatty Acid Thresholds, Fat Perception, Fatty Food Liking, and Papillae Density in Humans

Rivkeh Y. Haryono¹, Madeline A. Sprajcer¹, Russell S. J. Keast¹

¹School of Exercise and Nutrition Sciences, Deakin University

Oral chemoreception of fatty acids and the association with diet and fatty food preferences may enable the identification of mechanisms involved with the development of obesity and why dietary changes may be difficult for many individuals.

Genetics

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Sarah E. Ashley^1,2, Braydon A. Meyer^2,3, Justine A. Ellis^2,3,4, David J. Martino^2,3,5

¹Molecular Genetics of Chronic Inflammation and Allergic Disease, Max-Delbrück Center for Molecular Medicine, ²Murdoch Childrens Research Institute, ³Department of Paediatrics, University of Melbourne, ⁴Centre for Social and Early Emotional Development, Faculty of Health, Deakin University, ⁵Department of Paediatrics, University of Western Australia

Identification of genetic variants contributing to complex human disease allows us to identify novel mechanisms. Here, we demonstrate a multiplex genotyping approach to candidate genes or gene pathway analysis that maximizes the coverage at low cost and is amenable to cohort-based studies.

Medicine

Non-invasive Assessment of Dorsiflexor Muscle Function in Mice

Frederico Gerlinger-Romero¹, Alex B. Addinsall², Richard M. Lovering³, Victoria C. Foletta⁴, Chris van der Poel⁵, Paul A. Della-Gatta⁴, Aaron P. Russell⁴

¹School of Exercise and Nutrition Sciences, Deakin University, ²Centre for Molecular and Medical Research, School of Medicine, Deakin University, ³Department of Orthopaedics, School of Medicine, University of Maryland, ⁴Institute for Physical Activity and Nutrition (IPAN), School of Exercise and Nutrition Sciences, Deakin University, ⁵Department of Physiology, Anatomy and Microbiology, La Trobe University

Measurement of rodent skeletal muscle contractile function is a useful tool that can be used to track disease progression as well as efficacy of therapeutic intervention. We describe here the non-invasive, in vivo assessment of the dorsiflexor muscles that can be repeated over time in the same mouse.

Cancer Research

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Maryam Nakhjavani^1,2, Breanna Giles^1,2, Mia Strom^1,2, Chris Vi^1,2, Sahara Attenborough^1,2, Sarah Shigdar^1,2

¹School of Medicine, Deakin University, ²Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University

A necessary step in anticancer aptamer development is to test its binding to the target. We demonstrate a flow cytometric-based assay to study this binding, emphasizing the importance of including a negative control aptamer and cancer cells that are positive or negative for that particular protein.