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Umea University

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

Birendra Singh^1,2, Maryam Mostajeran¹, Yu-Ching Su¹, Tamim Al-Jubair¹, Kristian Riesbeck¹

¹Clinical Microbiology, Department of Translational Medicine, Lund University, ²Department of Molecular Biology, Umea University

This report describes protocols for characterizing interactions between bacterial outer membrane proteins and the human complement regulator vitronectin. The protocols can be used to study the binding reactions and biological function of vitronectin in any bacterial species.