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UT Health Science Center at San Antonio

3 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Ziming Cheng¹, Ting Zhou^1,2, Azhar Merchant¹, Thomas J. Prihoda³, Brian L. Wickes^4,5, Guogang Xu², Christi A. Walter^2,5, Vivienne I. Rebel^1,2,5

¹Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, ²Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, ³Department of Pathology, UT Health Science Center at San Antonio, ⁴Department of Microbiology, UT Health Science Center at San Antonio, ⁵Cancer Therapy and Research Center, UT Health Science Center at San Antonio

Here we describe an in vivo mutagenesis assay for small numbers of purified hematopoietic cells using the LacI transgenic mouse model. The LacI gene can be isolated to determine the frequency, location, and type of DNA mutants spontaneously arisen or after exposure to genotoxins.

Medicine

Evaluation of Capnography Sampling Line Compatibility and Accuracy when Used with a Portable Capnography Monitor

Ruben D. Restrepo¹, Ido Karpenkop², Katherine E. Liu³

¹Division of Respiratory Care, UT Health Science Center at San Antonio, ²Research and Development, OEM Engineering, Patient Monitoring, Medtronic, Jerusalem, ³Minimally Invasive Therapies Group, Scientific Communications, Medtronic, Minneapolis

The goal of this study was to evaluate the accuracy of capnography sampling lines used in conjunction with a portable bedside capnography monitor. Sampling lines from 7 manufacturers were evaluated for tensile strength, rise time, and ETCO₂ accuracy as a function of respiratory rate or supplemental oxygen flow rate.

JoVE Core

Quality-Controlled Sputum Analysis by Flow Cytometry

Marcia Grayson^*1, Shao-Chiang Lai^*1, Lydia H. Bederka¹, Patricia Araujo¹, Jamila Sanchez¹, Xavier T. Reveles¹, Vivienne I. Rebel^1,2, Jennifer Rebeles¹

¹bioAffinity Technologies, ²Department of Cell Systems & Anatomy, The University of Texas Health Science Center at San Antonio

This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.