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LSU School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

Prachi Jain¹, Rebecca A. Worthylake², Suresh K. Alahari^1,3

¹Department of Biochemistry and Molecular Biology, LSU School of Medicine, ²Department of Oral Biology, LSU School of Dentistry, ³Stanley S. Scott Cancer Center, LSU School of Medicine

This method allows monitoring of cells in real time and quantitative measurements of different cell migration parameters such as speed, displacement, and velocity. Unlike the traditional methods, this real time approach is not based on endpoint quantitative migration measurements; instead it allows monitoring and calculating different parameters continuously.

Medicine

Primary Tumor and MEF Cell Isolation to Study Lung Metastasis

Shengli Dong^*1, Mazvita Maziveyi^*1, Suresh K. Alahari¹

¹Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center

The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.