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Universität Würzburg

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Claudia Siverino¹, Barbara Tabisz², Tessa Lühmann³, Lorenz Meinel³, Thomas Müller⁴, Heike Walles^1,2, Joachim Nickel^1,2

¹Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg 'Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung', Institutsteil Würzburg, ²Lehrstuhl für Tissue Engineering und Regenerative Medizin, Universitätsklinikum Würzburg, ³Lehrstuhl für Pharmazeutische Technologie und Biopharmazie, Universität Würzburg, ⁴Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg

Biomaterials doped with Bone Morphogenetic Protein 2 (BMP2) have been used as a new therapeutic strategy to heal non-union bone fractures. To overcome side effects resulting from an uncontrollable release of the factor, we propose a new strategy to site-directly immobilize the factor, thus creating materials with improved osteogenic capabilities.

Neuroscience

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

Luca Zaeck¹, Madlin Potratz¹, Conrad M. Freuling¹, Thomas Müller¹, Stefan Finke¹

¹Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health

Novel, immunostaining-compatible tissue clearing techniques like the ultimate 3D imaging of solvent-cleared organs allow the 3D visualization of rabies virus brain infection and its complex cellular environment. Thick, antibody-labeled brain tissue slices are made optically transparent to increase imaging depth and to enable 3D analysis by confocal laser scanning microscopy.