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JoVE Journal

Neuroscience

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High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

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09:42 min

April 30th, 2019

April 30th, 2019

10,726 Views

0:04

Title

0:36

Brain Tissue Sample Fixation and Sectioning

1:26

Sample Pretreatment with Methanol

2:23

Immunostaining

4:03

Nuclear Staining and Tissue Clearing

5:08

Sample Mounting

6:08

Imaging and Image Processing

7:50

Results: Representative Deep Tissue Staining of Rabies Virus-Infected Brain Tissue Samples

9:08

Conclusion

필기록

This protocol enables the visualization of virus infection and provides deep insights into spatiotemporal resolution of the infection environment and its surrounding cellular context. The applicability of immunostaining to deep tissue imaging not

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Novel, immunostaining-compatible tissue clearing techniques like the ultimate 3D imaging of solvent-cleared organs allow the 3D visualization of rabies virus brain infection and its complex cellular environment. Thick, antibody-labeled brain tissue slices are made optically transparent to increase imaging depth and to enable 3D analysis by confocal laser scanning microscopy.

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