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University of Medicine and Dentistry of New Jersey - UMDNJ

4 ARTICLES PUBLISHED IN JoVE

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Biology

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro
Robert Clarke 1, Jianfeng Wang 1, Ellen Townes-Anderson 1
1Department of Neurology and Neuroscience, School of Medicine, University of Medicine and Dentistry of New Jersey - UMDNJ

This video describes the manipulation of cultured neurons using laser tweezers in vitro.

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Neuroscience

Organotypic Culture of Full-thickness Adult Porcine Retina
Jianfeng Wang 1, Anton M. Kolomeyer 2, Marco A. Zarbin 2, Ellen Townes-Anderson 1
1Neurology and Neurosciences, University of Medicine and Dentistry of New Jersey - UMDNJ, 2Institute of Ophthalmology and Visual Science, University of Medicine and Dentistry of New Jersey - UMDNJ

Here we describe a cost-effective technique for organotypic culture of adult porcine retina for seven days. Briefly, a sterile filter paper was used to lift the neural retina off from the RPE and place photoreceptor side up on an insert raised by a custom-made stand.

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Bioengineering

A Versatile Method of Patterning Proteins and Cells
Anil B. Shrirao *1, Frank H. Kung *2, Derek Yip 3, Bonnie L. Firestein 2, Cheul H, Cho 3, Ellen Townes-Anderson 4
1Department of Biomedical Engineering, Rutgers University, 2Department of Cell Biology and Neuroscience, Rutgers University, 3Department of Biomedical Engineering, New Jersey Institute of Technology, 4Department of Pharmacology, Physiology, and Neuroscience, Rutgers New Jersey Medical School

This report describes a simple, easy to perform technique, using low pressure vacuum, to fill microfluidic channels with cells and substrates for biological research.

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Education

Visualization of Failure and the Associated Grain-Scale Mechanical Behavior of Granular Soils under Shear using Synchrotron X-Ray Micro-Tomography
Zhuang Cheng 1, Jianfeng Wang 1,2
1Department of Architecture and Civil Engineering, City University of Hong Kong, 2Shenzhen Research Institute of City University of Hong Kong

The protocol describes procedures to acquire high-spatial resolution computed tomography (CT) images of a granular soil during triaxial compression, and to apply image processing techniques to these CT images to explore the grain-scale mechanical behavior of the soil under loading.

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