University of Medicine and Dentistry of New Jersey - UMDNJ

4 ARTICLES PUBLISHED IN JoVE

Biology

Using Laser Tweezers For Manipulating Isolated Neurons In Vitro

Robert Clarke¹, Jianfeng Wang¹, Ellen Townes-Anderson¹

¹Department of Neurology and Neuroscience, School of Medicine, University of Medicine and Dentistry of New Jersey - UMDNJ

This video describes the manipulation of cultured neurons using laser tweezers in vitro.

Neuroscience

Organotypic Culture of Full-thickness Adult Porcine Retina

Jianfeng Wang¹, Anton M. Kolomeyer², Marco A. Zarbin², Ellen Townes-Anderson¹

¹Neurology and Neurosciences, University of Medicine and Dentistry of New Jersey - UMDNJ, ²Institute of Ophthalmology and Visual Science, University of Medicine and Dentistry of New Jersey - UMDNJ

Here we describe a cost-effective technique for organotypic culture of adult porcine retina for seven days. Briefly, a sterile filter paper was used to lift the neural retina off from the RPE and place photoreceptor side up on an insert raised by a custom-made stand.

Bioengineering

A Versatile Method of Patterning Proteins and Cells

Anil B. Shrirao^*1, Frank H. Kung^*2, Derek Yip³, Bonnie L. Firestein², Cheul H, Cho³, Ellen Townes-Anderson⁴

¹Department of Biomedical Engineering, Rutgers University, ²Department of Cell Biology and Neuroscience, Rutgers University, ³Department of Biomedical Engineering, New Jersey Institute of Technology, ⁴Department of Pharmacology, Physiology, and Neuroscience, Rutgers New Jersey Medical School

This report describes a simple, easy to perform technique, using low pressure vacuum, to fill microfluidic channels with cells and substrates for biological research.

JoVE Core

Visualization of Failure and the Associated Grain-Scale Mechanical Behavior of Granular Soils under Shear using Synchrotron X-Ray Micro-Tomography

Zhuang Cheng¹, Jianfeng Wang^1,2

¹Department of Architecture and Civil Engineering, City University of Hong Kong, ²Shenzhen Research Institute of City University of Hong Kong

The protocol describes procedures to acquire high-spatial resolution computed tomography (CT) images of a granular soil during triaxial compression, and to apply image processing techniques to these CT images to explore the grain-scale mechanical behavior of the soil under loading.