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Barrow Neurological Institute

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

Rebecca L. McCall¹, Rachael W. Sirianni¹

¹Barrow Brain Tumor Research Center, Barrow Neurological Institute

We describe the production and characterization of nanoparticles and microparticles composed of poly(lactic-co-glycolic acid) using vitamin E-TPGS as an emulsifier. By varying formulation parameters such as the concentration of emulsifier, it is possible to produce nanoparticles with mean diameters ranging from 220 nm to 1.98 µm.

Neuroscience

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice

Rocio Leal-Campanario^1,2, Susana Martinez-Conde^2,3, Stephen L. Macknik^2,3

¹División de Neurociencias, Universidad Pablo de Olavide, ²Barrow Neurological Institute, ³Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center

Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice.