Rat Liver Perfusion and Primary Hepatocytes Isolation: An Old Procedure Crucial for Cutting-Edge 3D Organoids CultureValentina Tiriticco 1, Gabriele Codotto 1,2, Benedetta Blarasin 1,2, Noel Salvoza 1,2, Marco Stebel 2, Claudio Tiribelli 1, Cristina Bellarosa 1
1Italian Liver Foundation, 2Department of Life Sciences, University of Trieste
This protocol presents an optimized two-step collagenase liver perfusion technique in a rat model and shows the use of isolated hepatocytes for in vitro long-term culture of 3D organoids.
